The prevalence and genetical diversity of human Sapovirus were studied during an 18-month study in Ría do Burgo, an estuary nearby the city of A Coruña in Galicia (NW Spain). Sapovirus was detected using RT-qPCR procedure in 30 out of 80 mussel samples (37.5 %). Quantifications ranged from 2.2 × 10(3) to 2.1 × 10(5) RNA copies per gram of digestive tissue. Detection occurred mainly during the cold and rainy seasons of the period studied. Sequences obtained could be distributed into 5 genotypes being the most abundant GI.1 and GI.3. Results obtained indicate that the hydrodynamic characteristics of the harvesting area and the proximity of population density clearly influence the presence of the virus in shellfish.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12560-016-9242-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Viral gastroenteritis is a significant global health concern. An effective, rapid, and easy-to-use diagnostic tool is essential for screening causative viruses.
Methods: Forty-eight samples, known to be infected with one of the following viruses: norovirus, group A rotavirus, astrovirus, adenovirus, and sapovirus determined by reverse transcription-PCR and nucleotide sequencing, were evaluated by the Fast Track Diagnostics (FTD) viral gastroenteritis assay.
A Metagenomic Survey of Virological Hazards in Market-Ready Oysters.
Food Environ Virol
January 2025
Wageningen Food Safety Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands.
Viral contamination of bivalve molluscs, such as oysters, is a well-recognized food safety risk. The aim of this study was to assess virological hazards in market-ready oysters on the Dutch market. Non-targeted metagenome analysis was first performed on norovirus spiked-in samples showing linear and sensitive detection of norovirus GI.
View Article and Find Full Text PDFValidation and evaluation of the performance of QIAstat-Dx gastrointestinal panel in pooled stool samples.
Acta Trop
January 2025
Key Laboratory of Diarrhea Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, PR China. Electronic address:
In the current study, the analytical sensitivity, analytical specificity, reproducibility, anti-interferences ability, and clinical performance of the QIAstat-Dx Gastrointestinal Panel (GIP) system were evaluated using pooled stool samples. Results showed that the pooled sample test detected the selected ten targets exclusively, with no cross reaction with any other targets of common enteropathogens. The analytical sensitivity of the pooled sample test on QIAstat-Dx GIP system was 10 CFU/ml for Shigella spp.
View Article and Find Full Text PDFHuman Astrovirus Infection and Outbreaks in Japanese Children Before and After the Emergence of COVID-19 Pandemic.
J Med Virol
November 2024
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Article Synopsis
- A study analyzed 1,625 stool samples from children with diarrhea in Japan over four years (2018-2022) to detect human astroviruses (HAstVs) and other diarrheal viruses, finding HAstV in 8.6% of samples.
- The prevalence of HAstVs varied by year, with a notable increase to 20.1% in 2021-2022, and a significant infection rate was observed in children aged 1-3 years.
- The study identified multiple HAstV genotypes, including outbreaks of HAstV1 and MLB2 during the COVID-19 pandemic, and noted for the first time a mixed infection of HAstV1 and MLB1.
A dual typing system establishment and global diversity analysis for sapoviruses.
BMC Genomics
November 2024
College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Article Synopsis
- - The study focuses on refining the classification of sapoviruses, a diverse group of viruses causing gastroenteritis in humans and animals, by optimizing the criteria for their genogroups and genotypes due to an increase in identified sequences.
- - Using genetic distance analysis, researchers updated the classification thresholds, expanding VP1 genogroups to thirty-four and identifying twenty-four RdRp P-groups, leading to the addition of a new genotype for human sapoviruses.
- - The findings established a dual typing system for sapoviruses based on both VP1 and RdRp sequences, although no specific distribution pattern for human sapoviruses was detected during the analysis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!