Background: Mineral iron(III) recognition by bacteria is considered a matter of debate. The peculiar surface chemistry of novel naked magnetic nanoparticles, called SAMNs (surface active maghemite nanoparticles) characterized by solvent exposed Fe(3+) sites on their surface, was exploited for studying mineral iron sensing in Pseudomonas fluorescens.
Methods: SAMNs were applied for mimicking Fe(3+) ions in solution, acting as magnetically drivable probes to evaluate putative Fe(3+) recognition sites on the microorganism surface. Culture broths and nano-bio-conjugates were characterized by UV-Vis spectroscopy and mass spectrometry.
Results: The whole heritage of a membrane porin (OprF) of P. fluorescens Ps_22 cells was recognized and firmly bound by SAMNs. The binding of nanoparticles to OprF porin was correlated to a drastic inhibition of a siderophore (pyoverdine) biosynthesis and to the stimulation of the production and rate of formation of a secondary siderophore. The analysis of metabolic pathways, based on P. fluorescens Ps_22 genomic information, evidenced that this putative secondary siderophore does not belong to a selection of the most common siderophores.
Conclusions: In the scenario of an adhesion mechanism, it is plausible to consider OprF as the biological component deputed to the mineral iron sensing in P. fluorescens Ps_22, as well as one key of siderophore regulation.
General Significance: The present work sheds light on mineral iron sensing in microorganisms. Peculiar colloidal naked iron oxide nanoparticles offer a useful approach for probing the adhesion of bacterial surface on mineral iron for the identification of the specific recognition site for this iron uptake regulation in microorganisms.
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http://dx.doi.org/10.1016/j.bbagen.2016.05.007 | DOI Listing |
Water Res
January 2025
Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, China. Electronic address:
Anoxygenic photosynthetic bacteria (APB) have been frequently detected as a photoautotrophic Fe-carbon cycling drivers in photic and anoxic environment. However, the potential capacity of these bacteria for photoheterotrophic extracellular reduction of iron-containing minerals and their impact on the transformation of organic pollutants remain currently unknown. This study investigated the capacity of R.
View Article and Find Full Text PDFWater Res
December 2024
School of Environmental Studies, China University of Geosciences, Wuhan 430074, China; Hubei Key Laboratory of Yangtze Catchment Environmental, Aquatic Science, China University of Geosciences, Wuhan 430074, China. Electronic address:
An increasing number of studies have reported the coexistence of arsenic (As) and phosphorus at high concentrations in groundwater, which threatens human health and increases the complexity of groundwater remediation. However, limited work has been done regarding As interception in the presence of phosphate in flowing systems. In this study, a series of experiments were conducted to evaluate the interactions between phosphate and As during As removal by iron (Fe)-based biochar (FeBC).
View Article and Find Full Text PDFActa Pharm
December 2024
University of Zagreb Faculty of Pharmacy and Biochemistry 10000 Zagreb, Croatia.
Zeolites are a large family of minerals and the most studied is the naturally occurring clinoptilolite. They possess anti-inflammatory, antioxidant, and detoxifying properties which makes them valuable for medicinal use. Element analysis of zeolite's composition is necessary for its precise chemical characterization, and within this work development of a suspension method for the determination of manga nese, iron, and zinc by total reflection X-ray fluorescence spec-trometry (TXRF) was presented.
View Article and Find Full Text PDFFood Res Int
January 2025
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil. Electronic address:
Umbu-caja and soursop from the Northeast region of Brazil are rich in nutrients and bioactive compounds and are widely processed by the fruit agroindustry. However, there is a lack of research examining the composition and nutritional/technological potential of these co-product fruits. The present study evaluated the nutritional and technological characteristics of umbu-caja and soursop co-product flours (UCF and SCF, respectively), in addition to cytotoxicity in healthy cells.
View Article and Find Full Text PDFCarbohydr Polym
March 2025
School of Environmental and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:
Carbon dots (CDs) mediated g-CN (CN) is a promising visible-light-driven semiconductor in catalyzing peroxymonosulfate (PMS) for aqueous contaminants remediation. However, the poor dispersibility of powered catalyst and its challenging recyclability impede their broader application. Herein, we embedded FeN bridge within the g-CN framework and immobilized g-CN gel beads (CA/FNCCN) through a 3D cross-linking process with sodium alginate.
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