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A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer. | LitMetric

AI Article Synopsis

  • Early detection of gastrointestinal cancers can be enhanced through new molecular probes and imaging technologies, which include adapting a clinical fiberscope for wide-field fluorescence imaging while maintaining its white-light functionality.
  • The research developed a fluorescent fiberscope that uses a specialized probe, BMV109, to detect cancer-related signals linked to cathepsin proteases in tumor environments, validated through endoscopic exams in mice.
  • Results showed that the fiberscope effectively highlighted cancerous polyps in mice, suggesting its potential for early cancer detection in challenging areas during endoscopic procedures.

Article Abstract

Purpose: Early and effective detection of cancers of the gastrointestinal tract will require novel molecular probes and advances in instrumentation that can reveal functional changes in dysplastic and malignant tissues. Here, we describe adaptation of a wide-field clinical fiberscope to perform wide-field fluorescence imaging while preserving its white-light capability for the purpose of providing wide-field fluorescence imaging capability to point-of-care microscopes.

Procedures: We developed and used a fluorescent fiberscope to detect signals from a quenched probe, BMV109, that becomes fluorescent when cleaved by, and covalently bound to, active cathepsin proteases. Cathepsins are expressed in inflammation- and tumor-associated macrophages as well as directly from tumor cells and are a promising target for cancer imaging. The fiberscope has a 1-mm outer diameter enabling validation via endoscopic exams in mice, and therefore we evaluated topically applied BMV109 for the ability to detect colon polyps in an azoxymethane-induced colon tumor model in mice.

Results: This wide-field endoscopic imaging device revealed consistent and clear fluorescence signals from BMV109 that specifically localized to the polypoid regions as opposed to the normal adjacent colon tissue (p < 0.004) in the murine colon carcinoma model.

Conclusions: The sensitivity of detection of BMV109 with the fluorescence fiberscope suggested utility of these tools for early detection at hard-to-reach sites. The fiberscope was designed to be used in conjunction with miniature, endoscope-compatible fluorescence microscopes for dual wide-field and microscopic cancer detection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564400PMC
http://dx.doi.org/10.1007/s11307-016-0956-7DOI Listing

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