TNF-receptor associated factor (TRAF) proteins are key adaptor molecules containing E3 ubiquitin ligase activity that play a critical role in immune cell signaling. TRAF1 is a unique family of TRAF lacking the N-terminal RING finger domain. TRAF1 is an important scaffold protein that participates in TNFR2 signaling in T cells as a negative or positive regulator via direct interaction with TRAF2, which has recently been identified as a pro-apoptotic regulator in neuronal cell death. Here, we report the first crystal structure of the TRAF1 TRAF domain containing both the TRAF-N coiled-coil domain and the TRAF-C domain. Our structure reveals both similarities and differences with other TRAF family members, which may be functionally relevant to TRAFs. We also found that the TRAF-N coiled-coil domain of TRAF1 is critical for the trimer formation and stability of the protein. Finally, we found that conserved surface residues on the TRAF1 TRAF domain that might be binding hot spots that are critical for interaction with signaling molecules.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858697 | PMC |
| http://dx.doi.org/10.1038/srep25526 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sheep () Milk Exosomal miRNAs Attenuate Dextran Sulfate Sodium-Induced Colitis in Mice via TLR4 and TRAF-1 Inhibition.
J Agric Food Chem
September 2024
Laboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, China.
Article Synopsis
- Mammalian milk exosomal miRNAs, like those found in sheep milk, have been shown to help maintain gut immunity and protect the intestinal barrier, but the specific mechanisms are still under study.
- The research identified two key miRNAs (oar-miR-148a and oar-let-7b) in sheep milk-derived exosomes that target important immune receptors (TLR4 and TRAF1), using experiments to confirm their effects.
- In mice with chemically induced colitis, administering these sheep exosomes improved symptoms and reduced inflammation by altering the expression of TLR4 and TRAF1, suggesting a potential therapeutic role for these miRNAs in treating colitis.
Members of the TRAF gene family in Octopus sinensis and their response to PGN, poly I:C, and Vibrio parahaemolyticus.
Fish Shellfish Immunol
November 2024
State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361021, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China. Electronic address:
Article Synopsis
- Octopus sinensis is a significant species in aquaculture, particularly in southern China, and has a well-documented immune system that helps resist pathogens.
- Five TRAF genes (OsTRAF2 to OsTRAF7) were identified, each varying in size and showing different chromosome locations, indicating their role in the innate immune response.
- Analysis revealed that these genes are highly expressed in specific organs and their expression increases in response to immune challenges, suggesting they are vital for immune function in this species.
Uncovering the Interaction between TRAF1 and MAVS in the RIG-I Pathway to Enhance the Upregulation of IRF1/ISG15 during Classical Swine Fever Virus Infection.
Cells
July 2024
College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China.
Classical swine fever (CSF) is caused by the classical swine fever virus (CSFV), which poses a threat to swine production. The activation of host innate immunity through linker proteins such as tumor necrosis factor receptor (TNF-R)-associated factor (TRAF) is crucial for the induction of the NF-κB pathway. Recent research has revealed the involvement of mitochondrial antiviral-signaling protein (MAVS) in the interaction with TRAF2, 3, 5, and 6 to activate both the NF-κB and IRF3 pathways.
View Article and Find Full Text PDFTRAF1 from a Structural Perspective.
Biomolecules
April 2024
College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea.
Tumor necrosis factor receptor-associated factor (TRAF) proteins play pivotal roles in a multitude of cellular signaling pathways, encompassing immune response, cell fate determination, development, and thrombosis. Their involvement in these processes hinges largely on their ability to interact directly with diverse receptors via the TRAF domain. Given the limited binding interface, understanding how specific TRAF domains engage with various receptors and how structurally similar binding interfaces of TRAF family members adapt their distinct binding partners has been the subject of extensive structural investigations over several decades.
View Article and Find Full Text PDF[Immune Regulation by TNF Receptor-associated Factor 5].
Yakugaku Zasshi
May 2024
Laboratory of Molecular Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama.
The tumor necrosis factor receptor (TNFR)-associated factor (TRAF) family of molecules are intracellular adaptors that regulate cellular signaling through members of the TNFR and Toll-like receptor superfamily. Mammals have seven TRAF molecules numbered sequentially from TRAF1 to TRAF7. Although TRAF5 was identified as a potential regulator of TNFR superfamily members, the in vivo function of TRAF5 has not yet been fully elucidated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!