Objective: To investigate the effect and mechanism of Factor Xa on the differentiation of Meg-01 cells into platelet-like particles.
Methods: The Meg-01 cells were used as experimental object, Factor Xa was used as agonist. Cell proliferation was detected by CCK-8 assay. The viability of platelet-like particles was analyzed by AlamaBlue kit. MAPK/ERK pathway and PI3K/AKT pathway were assayed by Western blot. The expression of CD41b was analyzed by Western blot and flow cytometry. Cell cycle and apoptosis were detected by flow cytometry.
Results: The Factor Xa (1 µg/ml) inhibited cell viability, induced apoptosis. Factor Xa triggered cell arrest at the G(2)/M stage and down-regulated the expression of SKP2. After Meg-01 cells were stimulated by Factor Xa, the expression of CD41b was up-regulated and the MAPK/ERK pathway and PI3K/AKT pathway were activated. The platelets-like particles stimulated by FXa activation were viable.
Conclusion: The Factor Xa maybe display some effect on the differentiation of megakaryocytes into platelets.
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| http://dx.doi.org/10.7534/j.issn.1009-2137.2016.02.040 | DOI Listing |
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