Proximity ligation assay (PLA) appears as a quick and easy technique to visualize within fixed cells the occurrence and in situ distribution of protein complexes. PLA has been validated to detect protein-protein interactions within the nuclear compartment. Here, we describe a protocol which allows the detection of interactions between A-type nuclear lamins and either LEM-domain proteins (such as emerin, integrated within the inner nuclear membrane, and LAP2α which accumulates within the nucleoplasm) or gene regulatory factors (e.g., the transcription factor SREBP1). The distinct amounts and patterns of PLA signals obtained for various complexes highlight the pertinence of using PLA to reveal in situ where and to which extent nuclear envelope proteins bind specific partners.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-3530-7_9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nanoparticles-Based Optical Chemosensors for Lead Acetate Sensing in Water: ZnO, ZnCeO, and ZnNdO.
J Fluoresc
January 2025
Materials Science Lab (1), Physics Department, Faculty of Science, Cairo University, Giza, Egypt.
This study reports the synthesis, characterization, and optical properties of ZnO, ZnCeO, and ZnNdO nanoparticles and their interactions with lead acetate solutions. X-ray diffraction (XRD) confirmed that the nanoparticles were synthesized in a single-phase hexagonal structure, with crystallite sizes of 12.48 nm, 50.
View Article and Find Full Text PDFBio-nanopore technology for biomolecules detection.
Adv Biotechnol (Singap)
December 2024
School of Food Science and Technology, State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Bio-nanopore technology holds great promise in biomacromolecule detection, with its high throughput and low cost positioning it as an ideal detection tool. This technology employs a unique detection mechanism that utilizes nanoscale pores to rapidly and sensitively convert biological molecules interactions into electrical signals, enabling real-time, single-molecule detection with exceptional sensitivity. This review focuses on the latest advancements in this technology across various domains, including DNA and RNA sequencing, protein detection, and small molecule identification.
View Article and Find Full Text PDFRoots to the rescue: how plants harness hydraulic redistribution to survive drought across contrasting soil textures.
Adv Biotechnol (Singap)
November 2024
Root-Soil Interaction, TUM School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.
Hydraulic redistribution (HR) is a critical ecological process whereby plant roots transfer water from wetter to drier soil layers, significantly impacting soil moisture dynamics and plant water and nutrient uptake. Yet a comprehensive understanding of the mechanism triggering HR and its influencing factors remains elusive. Here, we conducted a systematic meta-analysis to discuss the influence of soil conditions and plant species characteristics on HR occurrence.
View Article and Find Full Text PDFA Statistically-Robust Model of the Axomyelin Unit under Normal Physiologic Conditions with Application to Disease States.
ASN Neuro
January 2025
Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois, USA.
Despite tremendous progress in characterizing the myriad cellular structures in the nervous system, a full appreciation of the interdependent and intricate interactions between these structures is as yet unfulfilled. Indeed, few more so than the interaction between the myelin internode and its ensheathed axon. More than a half-century after the ultrastructural characterization of this axomyelin unit, we lack a reliable understanding of the physiological properties, the significance and consequence of pathobiological processes, and the means to gauge success or failure of interventions designed to mitigate disease.
View Article and Find Full Text PDFDiscovery of viruses and bacteria associated with swine respiratory disease on farms at a nationwide scale in China using metatranscriptomic and metagenomic sequencing.
mSystems
January 2025
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Respiratory disease (RD) is a worldwide leading threat to the pig industry, but there is still limited understanding of the pathogens associated with swine RD. In this study, we conducted a nationwide genomic surveillance on identifying viruses, bacteria, and antimicrobial resistance genes (ARGs) from the lungs of pigs with RD in China. By performing metatranscriptomic sequencing combined with metagenomic sequencing, we identified 21 viral species belonging to 12 viral families.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!