Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution.
Genome Res
March 2025
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA;
Gene fusions are found as cancer drivers in diverse adult and pediatric cancers. Accurate detection of fusion transcripts is essential in cancer clinical diagnostics and prognostics and for guiding therapeutic development. Most currently available methods for fusion transcript detection are compatible with Illumina RNA-seq involving highly accurate short-read sequences.
View Article and Find Full Text PDFEngineering a cascaded isothermal signal amplification network using dimeric-palindromic primers for label-free detection of terminal deoxynucleotidyl transferase activity.
Talanta
March 2025
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address:
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase essential for the human immune system, is highly active in leukemic cells, making it a key biomarker for leukemia. However, existing TdT detection methods are often complex, costly, and radioactive. This study presents a novel, label-free approach for detecting TdT activity using a cascaded isothermal signal amplification network with high efficiency.
View Article and Find Full Text PDFCombining multiple physical fields to achieve on-demand signal conversion is essential. Optical manipulation based on holography, especially electric field-driven holography, can promote efficient information processing. However, there is a lack of a platform that can effectively connect optical recording/reading with electric field.
View Article and Find Full Text PDFMitochondria‑derived peptides: Promising microproteins in cardiovascular diseases (Review).
Mol Med Rep
May 2025
School of Basic Medicine Sciences, Shandong Second Medical University, Weifang, Shandong 261053, P.R. China.
Mitochondria‑derived peptides (MDPs) are a unique class of peptides encoded by short open reading frames in mitochondrial DNA, including the mitochondrial open reading frame of the 12S ribosomal RNA type‑c (MOTS‑c). Recent studies suggest that MDPs offer therapeutic benefits in various diseases, including neurodegenerative disorders and types of cancer, due to their ability to increase cellular resilience. Mitochondrial dysfunction is a key factor in the onset and progression of cardiovascular diseases (CVDs), such as atherosclerosis and heart failure, as it disrupts energy metabolism, increases oxidative stress and promotes inflammation.
View Article and Find Full Text PDFShort Read Lengths Recover Ecological Patterns in 16S rRNA Gene Amplicon Data.
Mol Ecol Resour
March 2025
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany.
16S rRNA gene metabarcoding, the study of amplicon sequences of the 16S rRNA gene from mixed environmental samples, is an increasingly popular and accessible method for assessing bacterial communities across a wide range of environments. As metabarcoding sequence data archives continue to grow, data reuse will likely become an important source of novel insights into the ecology of microbes. While recent work has demonstrated the benefits of longer read lengths for the study of microbial communities from 16S rRNA gene segments, no studies have explored the use of shorter (< 200 bp) read lengths in the context of data reuse.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!