Objective: To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science.
Methods: A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population.
Results: A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples.
Conclusion: The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
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Technical note: Y-chromosome short tandem repeat inconsistent typing for loci Y_GATA_H4, DYS481, DYS444, DYS635, DYS437, DYS533 and DYS570.
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View Article and Find Full Text PDFImproved understanding of sequence polymorphisms at 42 Y chromosome short tandem repeats for the Chinese Han population.
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February 2025
Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; School of Forensic Medicine, Kunming Medical University, Kunming 650500, China; School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, China. Electronic address:
Article Synopsis
- * Targeted sequencing of 42 Y-STR loci from 331 Chinese Han males revealed 387 sequence allele types and identified unique mutations and alleles that weren't previously recorded.
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