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Low background electrochemical sensor based on HCR towards acute myocardial infarction-specific miRNA detection.
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January 2025
Research Center for Bioengineering and Sensing Technology, Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China.
Acute myocardial infarction (AMI) accounts for a significant proportion of global fatalities, and early detection is crucial for improving patient outcomes. However, current diagnostic methods often struggle to detect AMI in its early stages. Herein, we present an electrochemical sensor utilizing a fractal gold (FracAu) electrode and hybridization chain reaction (HCR) amplification technology to detect AMI-specific microRNAs (miRNAs).
View Article and Find Full Text PDFThe revelation of genomic breed composition using target capture sequencing: a case of .
For Res (Fayettev)
October 2024
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, Sichuan, PR China.
plants have good flood tolerance and thus were introduced into China from North America in the early 1900s. The subsequent decades of cross-breeding experiments within have produced many new hybrid cultivars in China while also creating confusion in the genetic background of plants. In the present study, target capture sequencing-derived SNP markers were used to reveal the genomic composition of different species and cultivars of .
View Article and Find Full Text PDFPremise: Target sequence capture (Hyb-Seq) is a cost-effective sequencing strategy that employs RNA probes to enrich for specific genomic sequences. By targeting conserved low-copy orthologs, Hyb-Seq enables efficient phylogenomic investigations. Here, we present Asparagaceae1726-a Hyb-Seq probe set targeting 1726 low-copy nuclear genes for phylogenomics in the angiosperm family Asparagaceae-which will aid the often-challenging delineation and resolution of evolutionary relationships within Asparagaceae.
View Article and Find Full Text PDFTangled webs and spider-flowers: Phylogenomics, biogeography, and seed morphology inform the evolutionary history of Cleomaceae.
Am J Bot
September 2024
School of Biological Sciences, Washington State University, Pullman, Washington, 99164-4236, USA.
Premise: Cleomaceae is an important model clade for studies of evolutionary processes including genome evolution, floral form diversification, and photosynthetic pathway evolution. Diversification and divergence patterns in Cleomaceae remain tangled as research has been restricted by its worldwide distribution, limited genetic sampling and species coverage, and a lack of definitive fossil calibration points.
Methods: We used target sequence capture and the Angiosperms353 probe set to perform a phylogenetic study of Cleomaceae.
Phylogenomics of the pantropical Connaraceae: revised infrafamilial classification and the evolution of heterostyly.
Plant Syst Evol
August 2024
Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AE UK.
Article Synopsis
- Connaraceae is a diverse family of approximately 200 species, featuring unique floral traits like distyly and dioecy, but their internal relationships haven't been fully examined using modern molecular techniques.
- A phylogenomic study using Angiosperms353 probes analyzed nearly all genera from herbarium specimens, leading to a revised classification and insights into the evolution of their reproductive systems.
- The findings suggest tristyly may be the ancestral reproductive state for the family, though the complex nature of their floral diversity makes it difficult to draw definitive conclusions.
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