AI Article Synopsis
- Calcium is crucial for various processes in chloroplasts, but there's limited knowledge about calcium movement and binding proteins in plastids.
- A study analyzed the stroma of Arabidopsis chloroplasts to discover new calcium-binding proteins using 2D-PAGE and a calcium overlay assay.
- The research identified a small acidic protein called CP12, which can bind calcium and is important for regulating the Calvin-Benson-Bassham Cycle, suggesting that calcium signaling may influence carbon fixation.
Article Abstract
Calcium plays an important role in the regulation of several chloroplast processes. However, very little is still understood about the calcium fluxes or calcium-binding proteins present in plastids. Indeed, classical EF-hand containing calcium-binding proteins appears to be mostly absent from plastids. In the present study we analyzed the stroma fraction of Arabidopsis chloroplasts for the presence of novel calcium-binding proteins using 2D-PAGE separation followed by calcium overlay assay. A small acidic protein was identified by mass spectrometry analyses as the chloroplast protein CP12 and the ability of CP12 to bind calcium was confirmed with recombinant proteins. CP12 plays an important role in the regulation of the Calvin-Benson-Bassham Cycle participating in the assembly of a supramolecular complex between phosphoribulokinase and glyceraldehyde 3-phosphate dehydrogenase, indicating that calcium signaling could play a role in regulating carbon fixation.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844381 | PMC |
| http://dx.doi.org/10.3390/plants2030530 | DOI Listing |
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