The burden of cryptosporidiosis due to Cryptosporidium parvum is well documented in HIV-positive patients in Ethiopia. However, the role of animals in zoonotic transmission of the disease is poorly understood. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium species in dairy calves; to assess the role of cattle in zoonotic transmission in central Ethiopia. A total of 449 fecal samples were collected and screened using modified Ziehl-Neelson staining method and PCR targeting the small-subunit (SSU) rRNA gene. The prevalence of Cryptosporidium was 9.4% (42/449) and 15.8% (71/449) as detected by microscopy and nested PCR, respectively. The prevalence of infection varied significantly across the study areas with the higher prevalence being observed in Chancho 25.4% (30/118). Crossbred calves had significantly higher prevalence of Cryptosporidium than indigenous zebu. Genotyping results revealed the presence of C. andersoni (76.1%), C. bovis (19.7%) and C. ryanae (4.2%). The occurrence of these Cryptosporidium species appeared to be age-related. C. andersoni constituted 92.1% of the Cryptosporidium infection in calves older than 3 months. Sequence analysis also showed the existence of intra-species variation at SSU rRNA gene. Findings of the current study indicate that cattle may not be an important source of zoonotic cryptosporidiosis in central Ethiopia. Further molecular studies are needed to support this observation from other part of the country.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852944 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154647 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Microbial Safety of Sachet Water in Ghana: A Systematic Review.
Environ Health Insights
January 2025
Department of Medical Microbiology, University of Ghana Medical School, Korle Bu, Accra, Ghana.
Introduction: Access to safe drinking water is crucial for health and survival, yet many developing countries face significant challenges in this regard. In West Africa, rapid urbanisation has outpaced efforts to improve access to potable water, compelling households to rely on private vendors for solutions, particularly through the growing market for sachet water. Widely consumed in Ghana, sachet water has become a convenient and affordable option, with over 37% of the population depending on it.
View Article and Find Full Text PDFBackground: Globally, gastrointestinal (GI) infections are common, particularly in populations with low socioeconomic levels, including high illiteracy rates, ignorance, poor housing and lifestyles, and unfavorable environmental conditions. These risk factors are the underlying cause of GI parasitic infections in many developing nations, including Nepal.
Aim: This study aimed to assess the prevalence and diversity of GI parasites and their association with a few risk factors within the marginalized indigenous Tharu people of the southern region of Nepal.
Ozone disinfection of treated wastewater for inactivation of Cryptosporidium parvum for agricultural irrigation.
Water Environ Res
January 2025
Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo (CIAD), Culiacán, Sinaloa, Mexico.
The reliance on agriculture in many nations has increased the use of treated wastewater for irrigation. However, reclaimed water still poses health risks from resistant pathogens like Cryptosporidium spp. Ozone, a strong disinfectant, has been used in water treatment.
View Article and Find Full Text PDFUnlabelled: Once considered rare in eukaryotes, polycistronic mRNA expression has been identified in kinetoplastids and, more recently, green algae, red algae, and certain fungi. This study provides comprehensive evidence supporting the existence of polycistronic mRNA expression in the apicomplexan parasite . Leveraging long-read RNA-seq data from different parasite strains and using multiple long-read technologies, we demonstrate the existence of defined polycistronic transcripts containing 2-4 protein encoding genes, several validated with RT-PCR.
View Article and Find Full Text PDFPrevalence and Risk Factors of Waterborne and Foodborne Protozoan Pathogens in Kenya: A One Health Perspective.
China CDC Wkly
January 2025
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Intestinal infections affect approximately 450 million people globally, predominantly impacting children and immunocompromised individuals in low- and middle-income countries (LMICs) due to inadequate water, sanitation, and hygiene (WASH) conditions, poverty, malnutrition, and low literacy. In Kenya, the prevalence of intestinal infections is elevated by warm tropical climates and socioeconomic factors. This scoping review evaluates the national prevalence, risk factors, and contamination sources of intestinal protozoa in Kenya, using a One Health approach to synthesize existing data from various human, animal, and environmental studies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!