AI Article Synopsis
- The study aimed to evaluate the expression of microRNA-182 (miRNA-182) in human osteosarcoma (OS) cells and tissues, and its impact on tumor activity.
- Researchers used quantitative PCR to measure miRNA-182 levels, then manipulated its expression in MG-63 cells with mimics and inhibitors to study effects on cell viability, proliferation, and apoptosis.
- Results showed that miRNA-182 levels were significantly higher in OS cells compared to normal cells, and its upregulation led to increased apoptosis and reduced cell viability, indicating its potential role as a tumor suppressor gene in osteosarcoma.
Article Abstract
The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA-182 in human OS MG-63 cells, OS tissues and normal osteoblast hFOB1.19 cells was determined using quantitative polymerase chain reaction. Subsequently, a miRNA-182 mimic and inhibitor were utilized to regulate the expression level of this miRNA in MG-63 cells. Cell viability and proliferation were examined using cell counting kit-8 assays, and cell apoptosis was detected by flow cytometry. Cell invasion and migration assays were performed using Transwell chambers to analyze the biological functions of miRNA-182 . The present study demonstrated that the expression level of miRNA-182 in MG-63 cells and OS tissues was significantly increased compared with the hFOB1.19 cell line (P<0.05). The present study successfully performed cell transfections of miRNA-182 inhibitor and miRNA-182 mimic into MG-63 cells and achieved the desired transfection efficiency. The present study confirmed that upregulation of miRNA-182 promotes cell apoptosis and inhibits cell viability, proliferation, invasion and migration. The present findings additionally demonstrated that miRNA-182 is a tumor suppressor gene in OS. Therefore, regulating the expression of miRNA-182 may affect the biological behavior of OS cells, which suggests a potential role for miRNA-182 in molecular therapy for malignant tumors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840939 | PMC |
| http://dx.doi.org/10.3892/ol.2016.4375 | DOI Listing |
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