Objective: To establish a rapid, simple, and accurate method for measuring selenium in whole blood by atomic fluorescence spectrometry.
Methods: The whole blood samples were treated by microwave digestion and the acids were dispelled. After that the samples were pretreated with 50% hydrochloric acid as a reducing agent. Then the content of selenium in the pretreated samples was measured by hydride generation atomic fluorescence spectrometry.
Results: The calibration curve for selenium is linear in the range of 0~60 μg/L (r=0.9999). The detection limit, relative standard deviation, and recovery rate were 0.133 μg/L, 1.22%~2.08%, and 96.5%~101.8%, respectively.
Conclusion: This method is simple, with less consumption of chemical reagents, less pollution, and enough accuracy and sensitivity for determination of selenium in whole blood samples.
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| http://dx.doi.org/10.3760/cma.j.issn.1001-9391.2015.12.018 | DOI Listing |
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