AI Article Synopsis
- A new method for labeling carbon-13 in the yeast P. pastoris has been developed to enhance solid-state NMR studies of eukaryotic membrane proteins.
- The labeling combines natural methanol and specially labeled glycerol, improving spectral resolution by 1.5 times and creating specific labeling patterns.
- This approach is beneficial for gathering long-range distance information needed to determine the structures of large membrane proteins.
Article Abstract
We demonstrate a novel sparse (13)C labelling approach for methylotrophic yeast P. pastoris expression system, towards solid-state NMR studies of eukaryotic membrane proteins. The labelling scheme was achieved by co-utilizing natural abundance methanol and specifically (13)C labelled glycerol as carbon sources in the expression medium. This strategy improves the spectral resolution by 1.5 fold, displays site-specific labelling patterns, and has advantages for collecting long-range distance restraints for structure determination of large eukaryotic membrane proteins by solid-state NMR.
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| http://dx.doi.org/10.1007/s10858-016-0033-1 | DOI Listing |
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