The explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) has long been recognized as a problematic environmental pollutant, and efforts to remediate contaminated soils, sediments, and groundwater have been going on for decades. In recent years, much interest has focused on using bioremediation to clean up these sites. The current study investigated the microorganisms (16S rRNA genes, Illumina) and functional genes (xenA, xenB, and xplA) linked to RDX biodegradation in microcosms composed of sediment or groundwater from two Navy sites. For this, experiments included sediment samples from three depths (5 to 30 ft) from two wells located in one Navy site. In addition, the groundwater upstream and downstream of an emulsified oil biobarrier was examined from another Navy site. Further, for the groundwater experiments, the effect of glucose addition was explored. For the sediment experiments, the most enriched phylotypes during RDX degradation varied over time, by depth and well locations. However, several trends were noted, including the enrichment of Pseudomonas, Rhodococcus, Arthrobacter, and Sporolactobacillus in the sediment microcosms. For the groundwater-based experiments, Pseudomonas, unclassified Rhodocyclaceae, Sphingomonas, and Rhodococcus were also highly abundant during RDX degradation. The abundance of both xplA and xenA significantly increased during RDX degradation compared to the control microcosms for many treatments (both groundwater and sediment microcosms). In a limited number of microcosms, the copy number of the xenB gene increased. Phylotype data were correlated with functional gene data to highlight potentially important biomarkers for RDX biodegradation at these two Navy sites.
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http://dx.doi.org/10.1007/s00253-016-7559-8 | DOI Listing |
Chemosphere
December 2024
Anhui Province Key Laboratory of Polar Environment and Global Change, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China. Electronic address:
High concentrations of energetic compounds such as 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) in military-contaminated sites pose a serious threat to human health and ecosystems. Better understanding about their effects on microbial diversity and functional genes in soil of ammunition demolition sites is required. In this study, the information of soil microbial community composition was obtained by metagenomic sequencing, and the impacts of energetic compounds on microbial community structure at the level of functional genes and enzymes based on Nr (Non-Redundant Protein Sequence Database), KEGG (Kyoto Encyclopedia of Genes and Genomes), CAZy (Carbohydrate-Active enZymes Database) and other databases were discussed.
View Article and Find Full Text PDFJ Hazard Mater
November 2024
University of Delaware, Newark, DE 19716, USA.
Munition constituents (MC) in stormwater runoff have the potential to move these pollutants into receiving bodies at military installations. Here we present further evaluation of a passive and sustainable biofilter technology for removal of dissolved MC from simulated surface runoff by combined sorption-biodegradation processes under dynamic flow conditions. Columns were packed with MC sorbents Sphagnum peat moss and cationized (CAT) pine shavings with and without wood-based biochar.
View Article and Find Full Text PDFJ Environ Qual
November 2024
Biosphere 2, University of Arizona, Oracle, Arizona, USA.
Residue of energetic formulations, which is deposited on military training grounds following incomplete detonation, poses biotic hazards. This residue can be transported off-site, adsorb to soil clays and organic matter, transform or degrade, or taken up by plants and animals. Its harmful effects can be mitigated by localizing the energetics at the site of initial deposition using soil amendments and allowing them to bio- and photodegrade in situ.
View Article and Find Full Text PDFProg Neurobiol
November 2024
Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea; Department of Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Electronic address:
Ezrin-Radixin-Moesin (ERM) proteins are actin-binding proteins that contribute to morphological changes in dendritic spines. Despite their significant role in regulating spine structure, the role of ERM proteins in the nucleus accumbnes (NAcc) is not well known, especially in in the context of risk-reward decision-making. Here, we measured the relationship between synaptic excitation and inhibition (E/I ratio) from medium spiny neurons in the NAcc core obtained in the rat after a rat gambling task (rGT).
View Article and Find Full Text PDFDiagn Pathol
August 2024
Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600 077, India.
Background: Podoplanin (PDPN) is a transmembrane glycoprotein implicated in the pathogenesis of odontogenic lesions (OL). It is localized at the membrane and cytoplasmic level, and its interaction with other proteins could trigger cell proliferation, invasion and migration. The main objective of this systematic review is to explore the immunoexpression pattern of podoplanin in OL.
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