The administration of city of Serpukhov of the Moscow oblast, 142203 Serpukhov, Russia The article presents results of special study carried out with the purpose to determine effectiveness of the Program "Development of health care system of the city of Serpukhov in 2011-2013". The comparative analysis was applied to indicators planned by the Program as achievements and actually achieved indicators. The critical remarks concerning listing of regulate indicators are presented and argued.
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Viruses
January 2025
State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia.
is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were assessed using in vivo mouse sepsis and burn skin infection models. In the mouse sepsis model, in the case of prevention or early treatment, a single K9-specific phage or recombinant depolymerase injection was able to protect 100% of the mice after parenteral infection with a lethal dose of of the K9-type, with complete eradication of the pathogen.
View Article and Find Full Text PDFViruses
May 2024
State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia.
The genus comprises both environmental and clinically relevant species associated with hospital-acquired infections. Among them, is a critical priority bacterial pathogen, for which the research and development of new strategies for antimicrobial treatment are urgently needed. spp.
View Article and Find Full Text PDFInt J Mol Sci
February 2024
State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia.
Two novel virulent phages of the genus infecting , a significant nosocomial pathogen, have been isolated and studied. Phages Brutus and Scipio were able to infect strains belonging to the K116 and K82 capsular types, respectively. The biological properties and genomic organization of the phages were characterized.
View Article and Find Full Text PDFVirus Res
December 2022
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, City District Serpukhov, Moscow Region, Russia.
Bacteriophages and phage polysaccharide-degrading enzymes (depolymerases) are garnering attention as possible alternatives to antibiotics. Here, we describe the antimicrobial properties of bacteriophage KpV74 and phage depolymerase Dep_kpv74 specific to the hypervirulent Klebsiella pneumoniae of the K2 capsular type. The depolymerase Dep_kpv74 was identified as a specific glucosidase that cleaved the K2 type capsular polysaccharides of the K.
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August 2021
State Research Center for Applied Microbiology and Biotechnology, 24 "Quarter A" Territory, Obolensk, City District Serpukhov, 142279 Moscow, Russia.
appears to be one of the most crucial nosocomial pathogens. A possible component of antimicrobial therapy for infections caused by extremely drug-resistant strains may be specific lytic bacteriophages or phage-derived enzymes. In the present study, we observe the biological features, genomic organization, and phage-host interaction strategy of novel virulent bacteriophage Aristophanes isolated on strain having K26 capsular polysaccharide structure.
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