Methods to Study Plant Programmed Cell Death.

Methods Mol Biol

School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.

Published: December 2017

AI Article Synopsis

  • Programmed cell death (PCD) plays a vital role in plant growth, defense against pathogens, and adaptability to environmental challenges.
  • This chapter offers specific methods to effectively investigate PCD linked to plant development and stress responses.
  • Techniques like the root hair assay and electrolyte leakage assay allow for measuring PCD and cellular damage due to abiotic stress, while the lace plant serves as a model for examining genetic control of PCD in leaf development.

Article Abstract

Programmed cell death (PCD) is a critical component of plant development, defense against invading pathogens, and response to environmental stresses. In this chapter, we provide detailed technical methods for studying PCD associated with plant development or induced by abiotic stress. A root hair assay or electrolyte leakage assay are excellent techniques for the quantitative determination of PCD and/or cellular injury induced in response to abiotic stress, whereas the lace plant provides a unique model that facilitates the study of genetically regulated PCD during leaf development.

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Source
http://dx.doi.org/10.1007/978-1-4939-3581-9_12DOI Listing

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