Crystal Bioscience, Inc., Emeryville, California, United States of America.
Published: March 2017
AI Article Synopsis
Article Abstract
The CRISPR/Cas9 system has been applied in a large number of animal and plant species for genome editing. In chickens, CRISPR has been used to knockout genes in somatic tissues, but no CRISPR-mediated germline modification has yet been reported. Here we use CRISPR to target the chicken immunoglobulin heavy chain locus in primordial germ cells (PGCs) to produce transgenic progeny. Guide RNAs were co-transfected with a donor vector for homology-directed repair of the double-strand break, and clonal populations were selected. All of the resulting drug-resistant clones contained the correct targeting event. The targeted cells gave rise to healthy progeny containing the CRISPR-targeted locus. The results show that gene-edited chickens can be obtained by modifying PGCs in vitro with the CRISPR/Cas9 system, opening up many potential applications for efficient genetic modification in birds.
Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, 364012, Fujian, China. Electronic address:
* Phylogenetic studies categorize TMUV into four lineages, with Cluster 2 being the most adapted to duck codon usage patterns, highlighting the influence of natural selection on its evolution.
* The virus shows lower adaptation to potential hosts like humans and mosquitoes, and analyses reveal that its genome undergoes significant changes to avoid immune responses, aiding its fitness in poultry.
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea. Electronic address:
Multidrug-resistant strains, particularly those linked to farm animals, are a growing concern for human health due to their ability to cause severe intestinal and extraintestinal diseases.
This study focuses on APEC 36, a strain isolated from a chicken with a serious infection, analyzing its genome and finding it has multiple antibiotic resistance mechanisms, mainly antibiotic efflux.
APEC 36 also contains unique genetic traits, such as a rare beta-lactamase variant and genes linked to toxins and iron uptake, indicating that it could pose significant threats to human health.
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:
Adiponectin (ADPN) exerts various cellular and metabolic functions by activating signaling pathways, including extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) pathways, the protein kinase B (Akt) pathway, and the p38 mitogen-activated protein kinase (MAPK) pathway. However, generating functional recombinant human adiponectin (hADPN) in bacterial or mammalian cells is challenging. Although ADPN agonist peptides have been developed, problems like stability, solubility, and affinity for receptors remain.
Transgenic chicken bioreactors can efficiently produce egg whites containing large quantities of recombinant proteins. We previously developed transgenic chickens that produce recombinant monoclonal antibodies (mAbs) against epidermal growth factor receptor 2 (HER2). However, the practical applications of mAbs derived from transgenic eggs have not yet been examined.
