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<PubmedArticle><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27099637</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>21</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1874-2106</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>The open dentistry journal</Title><ISOAbbreviation>Open Dent J</ISOAbbreviation></Journal><ArticleTitle>Prevalence of &#x3b2;-lactam (bla TEM) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects.</ArticleTitle><Pagination><StartPage>89</StartPage><EndPage>98</EndPage><MedlinePgn>89-98</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2174/1874210601610010089</ELocationID><Abstract><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">The aim of this study is to investigate the prevalence of bla TEM and nim genes that encode resistance to &#x3b2;-lactams and nitroimidazoles, respectively, in the oral cavity of systemically healthy Greek subjects.</AbstractText><AbstractText Label="MATERIALS AND METHODOLOGY" NlmCategory="METHODS">After screening 720 potentially eligible subjects, 154 subjects were recruited for the study, including 50 periodontally healthy patients, 52 cases of gingivitis and 52 cases of chronic periodontitis. The clinical parameters were assessed with an automated probe. Various samples were collected from the tongue, first molars and pockets &gt;6mm, and analysed by polymerase chain reaction-amplification of the bla TEM and nim genes, using primers and conditions previously described in the literature.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">There was a high rate of detection of bla TEM in plaque and tongue samples alike in all periodontal conditions (37% of plaque and 60% of tongue samples, and 71% of participants). The bla TEM gene was detected more frequently in the tongue samples of the periodontally healthy (56%) and chronic periodontitis (62%) groups compared to the plaque samples from the same groups (36% and 29%, respectively; z-test with Bonferroni corrections-tests, P&lt;0.05). The nim gene was not detected in any of the 343 samples analysed.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The oral cavity of Greek subjects often harbours bla TEM but not nim genes, and therefore the antimicrobial activity of &#x3b2;-lactams might be compromised.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koukos</LastName><ForeName>Georgios</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>251 General Air Force Hospital, Department of Periodontology, Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Konstantinidis</LastName><ForeName>Antonios</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School, Aristotle University of Thessaloniki, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsalikis</LastName><ForeName>Lazaros</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School, Aristotle University of Thessaloniki, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arsenakis</LastName><ForeName>Minas</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Genetics and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Slini</LastName><ForeName>Theodora</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Mechanical Engineering, Aristotle University of Thessaloniki, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sakellari</LastName><ForeName>Dimitra</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School, Aristotle University of Thessaloniki, Greece.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United Arab Emirates</Country><MedlineTA>Open Dent J</MedlineTA><NlmUniqueID>101480503</NlmUniqueID><ISSNLinking>1874-2106</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">blaTEM gene</Keyword><Keyword MajorTopicYN="N">metronidazole</Keyword><Keyword MajorTopicYN="N">microbial resistance</Keyword><Keyword MajorTopicYN="N">nim gene</Keyword><Keyword MajorTopicYN="N">&#x3b2;-Lactams</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>2</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>11</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>11</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="pmc-release"><Year>2016</Year><Month>1</Month><Day>1</Day></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27099637</ArticleId><ArticleId IdType="pmc">PMC4820533</ArticleId><ArticleId IdType="doi">10.2174/1874210601610010089</ArticleId><ArticleId IdType="pii">TODENTJ-10-89</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>World Health Organization. The Evolving Threat of Antimicrobial Resistance: options for action. Geneva [cited 2015 August 13]. 2015.  Available from: http://whqlibdoc.who.int/publications/2012/9789241503181_eng.pdf .</Citation></Reference><Reference><Citation>Sweeney L.C., Dave J., Chambers P.A., Heritage J. Antibiotic resistance in general dental practice--a cause for concern? J. Antimicrob. Chemother. 2004;53(4):567&#x2013;576. doi: 10.1093/jac/dkh137.</Citation><ArticleIdList><ArticleId IdType="doi">10.1093/jac/dkh137</ArticleId><ArticleId IdType="pubmed">14985274</ArticleId></ArticleIdList></Reference><Reference><Citation>Fluit A.C., Visser M.R., Schmitz F.J. Molecular detection of antimicrobial resistance. Clin. Microbiol. Rev. 2001;14(4):836&#x2013;871. doi: 10.1128/CMR.14.4.836-871.2001.</Citation><ArticleIdList><ArticleId IdType="doi">10.1128/CMR.14.4.836-871.2001</ArticleId><ArticleId IdType="pmc">PMC89006</ArticleId><ArticleId IdType="pubmed">11585788</ArticleId></ArticleIdList></Reference><Reference><Citation>Roberts A.P., Pratten J., Wilson M., Mullany P. Transfer of a conjugative transposon, Tn5397 in a model oral biofilm. FEMS Microbiol. Lett. 1999;177(1):63&#x2013;66. doi: 10.1111/j.1574-6968.1999.tb13714.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1574-6968.1999.tb13714.x</ArticleId><ArticleId IdType="pubmed">10436923</ArticleId></ArticleIdList></Reference><Reference><Citation>Warburton P.J., Palmer R.M., Munson M.A., Wade W.G. Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon. J. Antimicrob. Chemother. 2007;60(5):973&#x2013;980. doi: 10.1093/jac/dkm331.</Citation><ArticleIdList><ArticleId IdType="doi">10.1093/jac/dkm331</ArticleId><ArticleId IdType="pubmed">17855723</ArticleId></ArticleIdList></Reference><Reference><Citation>Poole K. Resistance to &#x3b2;-lactam antibiotics. Cell. Mol. Life Sci. 2004;61(17):2200&#x2013;2223. doi: 10.1007/s00018-004-4060-9.</Citation><ArticleIdList><ArticleId IdType="doi">10.1007/s00018-004-4060-9</ArticleId><ArticleId IdType="pmc">PMC11138534</ArticleId><ArticleId IdType="pubmed">15338052</ArticleId></ArticleIdList></Reference><Reference><Citation>Chopra I., Roberts M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev. 2001;65(2):232&#x2013;260. doi: 10.1128/MMBR.65.2.232-260.2001.</Citation><ArticleIdList><ArticleId IdType="doi">10.1128/MMBR.65.2.232-260.2001</ArticleId><ArticleId IdType="pmc">PMC99026</ArticleId><ArticleId IdType="pubmed">11381101</ArticleId></ArticleIdList></Reference><Reference><Citation>Carlier J.P., Sellier N., Rager M.N., Reysset G. Metabolism of a 5-nitroimidazole in susceptible and resistant isogenic strains of Bacteroides fragilis. Antimicrob. Agents Chemother. 1997;41(7):1495&#x2013;1499.</Citation><ArticleIdList><ArticleId IdType="pmc">PMC163946</ArticleId><ArticleId IdType="pubmed">9210672</ArticleId></ArticleIdList></Reference><Reference><Citation>European Centre for Disease Prevention and Control. Antimicrobial resistance surveillance in Europe 2012. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). Stockholm [cited 2015 August 13]. 2012.  Available from: http://www.ecdc.europa.eu/en/publica-tions/Publications/antimicrobial-resistance-surveillance-europe-2012.pdf .</Citation></Reference><Reference><Citation>Tonetti M.S., Claffey N., European Workshop in Periodontology group C Advances in the progression of periodontitis and proposal of definitions of a periodontitis case and disease progression for use in risk factor research. Group C consensus report of the 5th European Workshop in Periodontology. J. Clin. Periodontol. 2005;32(Suppl. 6):210&#x2013;213. doi: 10.1111/j.1600-051X.2005.00822.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1600-051X.2005.00822.x</ArticleId><ArticleId IdType="pubmed">16128839</ArticleId></ArticleIdList></Reference><Reference><Citation>Osborn J., Stoltenberg J., Huso B., Aeppli D., Pihlstrom B. Comparison of measurement variability using a standard and constant force periodontal probe. J. Periodontol. 1990;61(8):497&#x2013;503. doi: 10.1902/jop.1990.61.8.497.</Citation><ArticleIdList><ArticleId IdType="doi">10.1902/jop.1990.61.8.497</ArticleId><ArticleId IdType="pubmed">2391627</ArticleId></ArticleIdList></Reference><Reference><Citation>Goncharoff P., Figurski D.H., Stevens R.H., Fine D.H. Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of lktA-specific sequences. Oral Microbiol. Immunol. 1993;8(2):105&#x2013;110. doi: 10.1111/j.1399-302X.1993.tb00554.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1399-302X.1993.tb00554.x</ArticleId><ArticleId IdType="pubmed">8355983</ArticleId></ArticleIdList></Reference><Reference><Citation>Livermore D.M. &#x3b2;-Lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 1995;8(4):557&#x2013;584.</Citation><ArticleIdList><ArticleId IdType="pmc">PMC172876</ArticleId><ArticleId IdType="pubmed">8665470</ArticleId></ArticleIdList></Reference><Reference><Citation>Trinh S., Reysset G. Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides spp. J. Clin. Microbiol. 1996;34(9):2078&#x2013;2084.</Citation><ArticleIdList><ArticleId IdType="pmc">PMC229193</ArticleId><ArticleId IdType="pubmed">8862561</ArticleId></ArticleIdList></Reference><Reference><Citation>Alsultan A.A., Aboulmagd E., Amin T.T. ESBL-producing E. coli and K. pneumoniae in Al-Ahsa, Saudi Arabia: antibiotic susceptibility and prevalence of blaSHV and blaTEM. J. Infect. Dev. Ctries. 2013;7(12):1016&#x2013;1019. doi: 10.3855/jidc.3764.</Citation><ArticleIdList><ArticleId IdType="doi">10.3855/jidc.3764</ArticleId><ArticleId IdType="pubmed">24334953</ArticleId></ArticleIdList></Reference><Reference><Citation>Ioannidis I., Sakellari D., Spala A., Arsenakis M., Konstantinidis A. Prevalence of tetM, tetQ, nim and bla(TEM) genes in the oral cavities of Greek subjects: a pilot study. J. Clin. Periodontol. 2009;36(7):569&#x2013;574. doi: 10.1111/j.1600-051X.2009.01425.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1600-051X.2009.01425.x</ArticleId><ArticleId IdType="pubmed">19538330</ArticleId></ArticleIdList></Reference><Reference><Citation>Fosse T., Madinier I., Hitzig C., Charbit Y. Prevalence of beta-lactamase-producing strains among 149 anaerobic gram-negative rods isolated from periodontal pockets. Oral Microbiol. Immunol. 1999;14(6):352&#x2013;357. doi: 10.1034/j.1399-302X.1999.140604.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1034/j.1399-302X.1999.140604.x</ArticleId><ArticleId IdType="pubmed">10895690</ArticleId></ArticleIdList></Reference><Reference><Citation>Rams T.E., Degener J.E., van Winkelhoff A.J. Antibiotic resistance in human chronic periodontitis microbiota. J. Periodontol. 2014;85(1):160&#x2013;169. doi: 10.1902/jop.2013.130142.</Citation><ArticleIdList><ArticleId IdType="doi">10.1902/jop.2013.130142</ArticleId><ArticleId IdType="pubmed">23688097</ArticleId></ArticleIdList></Reference><Reference><Citation>Walker C.B. The acquisition of antibiotic resistance in the periodontal microflora. Periodontology 2000. 1996;10(1):79&#x2013;88. doi: 10.1111/j.1600-0757.1996.tb00069.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1600-0757.1996.tb00069.x</ArticleId><ArticleId IdType="pubmed">9567938</ArticleId></ArticleIdList></Reference><Reference><Citation>van Winkelhoff A.J., Herrera D., Oteo A., Sanz M. Antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in The Netherlands and Spain. J. Clin. Periodontol. 2005;32(8):893&#x2013;898. doi: 10.1111/j.1600-051X.2005.00782.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1600-051X.2005.00782.x</ArticleId><ArticleId IdType="pubmed">15998275</ArticleId></ArticleIdList></Reference><Reference><Citation>Patel M. The prevalence of beta lactamase-producing anaerobic oral bacteria in South African patients with chronic periodontitis. SADJ. 2011;66(9):416&#x2013;418.</Citation><ArticleIdList><ArticleId IdType="pubmed">23193871</ArticleId></ArticleIdList></Reference><Reference><Citation>Rams T.E., Degener J.E., van Winkelhoff A.J. Prevalence of &#x3b2;-lactamase-producing bacteria in human periodontitis. J. Periodontal Res. 2013;48(4):493&#x2013;499. doi: 10.1111/jre.12031.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/jre.12031</ArticleId><ArticleId IdType="pubmed">23173872</ArticleId></ArticleIdList></Reference><Reference><Citation>Wilke M.S., Lovering A.L., Strynadka N.C. Beta-lactam antibiotic resistance: a current structural perspective. Curr. Opin. Microbiol. 2005;8(5):525&#x2013;533. doi: 10.1016/j.mib.2005.08.016.</Citation><ArticleIdList><ArticleId IdType="doi">10.1016/j.mib.2005.08.016</ArticleId><ArticleId IdType="pubmed">16129657</ArticleId></ArticleIdList></Reference><Reference><Citation>van Winkelhoff A.J., Winkel E.G., Barendregt D., Dellemijn-Kippuw N., Stijne A., van der Velden U. &#x3b2;eta-lactamase producing bacteria in adult periodontitis. J. Clin. Periodontol. 1997;24(8):538&#x2013;543. doi: 10.1111/j.1600-051X.1997.tb00226.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/j.1600-051X.1997.tb00226.x</ArticleId><ArticleId IdType="pubmed">9266340</ArticleId></ArticleIdList></Reference><Reference><Citation>Feres M., Haffajee A.D., Allard K., Som S., Goodson J.M., Socransky S.S. Antibiotic resistance of subgingival species during and after antibiotic therapy. J. Clin. Periodontol. 2002;29(8):724&#x2013;735. doi: 10.1034/j.1600-051X.2002.290809.x.</Citation><ArticleIdList><ArticleId IdType="doi">10.1034/j.1600-051X.2002.290809.x</ArticleId><ArticleId IdType="pubmed">12390569</ArticleId></ArticleIdList></Reference><Reference><Citation>Guerrero A., Nibali L., Lambertenghi R., Ready D., Suvan J., Griffiths G.S., Wilson M., Tonetti M.S. Impact of baseline microbiological status on clinical outcomes in generalized aggressive periodontitis patients treated with or without adjunctive amoxicillin and metronidazole: an exploratory analysis from a randomized controlled clinical trial. J. Clin. Periodontol. 2014;41(11):1080&#x2013;1089. doi: 10.1111/jcpe.12299.</Citation><ArticleIdList><ArticleId IdType="doi">10.1111/jcpe.12299</ArticleId><ArticleId IdType="pubmed">25139116</ArticleId></ArticleIdList></Reference><Reference><Citation>European Centre for Disease Prevention and Control. Surveillance of antimicrobial consumption in Europe, 2010. Stockholm [cited 2015 August 13]. 2010.  Available from: http://ecdc.europa.eu/en/publications/Publications/antimi-crobial-antibiotic-consumption-ESAC- report-2010-data.pdf .</Citation></Reference><Reference><Citation>Koukos G., Sakellari D., Arsenakis M., Tsalikis L., Slini T., Konstantinidis A. Prevalence of tetracycline resistance genes in the oral cavity of Greek subjects. J Biol Res-Thessalon. 2013;20:387&#x2013;394.</Citation></Reference><Reference><Citation>Trinh S., Reysset G. Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J. Bacteriol. 1997;179(12):4071&#x2013;4074.</Citation><ArticleIdList><ArticleId IdType="pmc">PMC179223</ArticleId><ArticleId IdType="pubmed">9190830</ArticleId></ArticleIdList></Reference><Reference><Citation>Alauzet C., Mory F., Teyssier C., Hallage H., Carlier J.P., Grollier G., Lozniewski A. Metronidazole resistance in Prevotella spp. and description of a new nim gene in Prevotella baroniae. Antimicrob. Agents Chemother. 2010;54(1):60&#x2013;64. doi: 10.1128/AAC.01003-09.</Citation><ArticleIdList><ArticleId IdType="doi">10.1128/AAC.01003-09</ArticleId><ArticleId IdType="pmc">PMC2798483</ArticleId><ArticleId IdType="pubmed">19805556</ArticleId></ArticleIdList></Reference><Reference><Citation>Abu-Fanas S.H., Drucker D.B., Hull P.S., Reeder J.C., Ganguli L.A. Identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets. J. Dent. 1991;19(1):46&#x2013;50. doi: 10.1016/0300-5712(91)90038-Z.</Citation><ArticleIdList><ArticleId IdType="doi">10.1016/0300-5712(91)90038-Z</ArticleId><ArticleId IdType="pubmed">1901873</ArticleId></ArticleIdList></Reference><Reference><Citation>Listgarten M.A., Lai C.H., Young V. Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. J. Periodontol. 1993;64(3):155&#x2013;161. doi: 10.1902/jop.1993.64.3.155.</Citation><ArticleIdList><ArticleId IdType="doi">10.1902/jop.1993.64.3.155</ArticleId><ArticleId IdType="pubmed">8463936</ArticleId></ArticleIdList></Reference><Reference><Citation>Stewart P.S., Costerton J.W. Antibiotic resistance of bacteria in biofilms. Lancet. 2001;358(9276):135&#x2013;138. doi: 10.1016/S0140-6736(01)05321-1.</Citation><ArticleIdList><ArticleId IdType="doi">10.1016/S0140-6736(01)05321-1</ArticleId><ArticleId IdType="pubmed">11463434</ArticleId></ArticleIdList></Reference><Reference><Citation>ten Cate J.M. Biofilms, a new approach to the microbiology of dental plaque. Odontology. 2006;94(1):1&#x2013;9. doi: 10.1007/s10266-006-0063-3.</Citation><ArticleIdList><ArticleId IdType="doi">10.1007/s10266-006-0063-3</ArticleId><ArticleId IdType="pubmed">16998612</ArticleId></ArticleIdList></Reference><Reference><Citation>Hausner M., Wuertz S. High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis. Appl. Environ. Microbiol. 1999;65(8):3710&#x2013;3713.</Citation><ArticleIdList><ArticleId IdType="pmc">PMC91555</ArticleId><ArticleId IdType="pubmed">10427070</ArticleId></ArticleIdList></Reference><Reference><Citation>Roschanski N., Fischer J., Guerra B., Roesler U. Development of a multiplex real-time PCR for the rapid detection of the predominant beta-lactamase genes CTX-M, SHV, TEM and CIT-type AmpCs in Enterobacteriaceae. PLoS One. 2014;9(7):e100956. doi: 10.1371/journal.pone.0100956.</Citation><ArticleIdList><ArticleId IdType="doi">10.1371/journal.pone.0100956</ArticleId><ArticleId IdType="pmc">PMC4102473</ArticleId><ArticleId IdType="pubmed">25033234</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></PubmedArticle></PubmedArticleSet>