AI Article Synopsis
- The study developed a method to simultaneously measure shikonin, acetyl shikonin, and β,β'-dimethylpropene shikonin in Onosma hookeri using HPLC-DAD with a specific gradient elution technique.
- Quality assessment of seven batches revealed significant variability in the three naphthoquinone constituents, with fingerprint analysis categorizing the samples into four distinct groups based on their similarity values.
- The findings suggest the complexity and inconsistency in the use of this medicinal plant, while the established HPLC fingerprints and quantitative analysis can effectively assess its quality.
Article Abstract
A method for simultaneous determination of the shikonin, acetyl shikonin and β, β'-dimethylpropene shikonin in Onosma hookeri and the chromatographic fingerprint was estabished by HPLC-DAD on an Agilent Zorbax SB-column with a gradient elution of acetonitrile and water at 0.8 mL x min(-1), 30 degrees C. The quality assessment was conducted by comparing the content difference of three naphthoquinone constituents, in combination with chromatographic fingerprint analysis and systems cluster analysis among 7 batches of radix O. hookeri. The content of the three naphthoquinone constituents showed wide variations in 7 bathces. The similarity value of the fingerprints of sample 5, 6 and 7 was above 0.99, sample 2 and 3 above 0.97, sample 3 and 4 above 0.90, and other samples larger than 0.8, which was in concert with the content of three naphthoquinone constituents. The 7 samples were roughly divided into 4 categories. The results above indicated that the using of this medicine is complex and rather spotty. The established HPLC fingerprints and the quantitative analysis method can be used efficiently for quality assessment of O. hookeri.
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