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CompositIA: an open-source automated quantification tool for body composition scores from thoraco-abdominal CT scans.
Eur Radiol Exp
January 2025
Imaging Institute of Southern Switzerland, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Background: Body composition scores allow for quantifying the volume and physical properties of specific tissues. However, their manual calculation is time-consuming and prone to human error. This study aims to develop and validate CompositIA, an automated, open-source pipeline for quantifying body composition scores from thoraco-abdominal computed tomography (CT) scans.
View Article and Find Full Text PDFIncreased FGF-19 levels following explantation in women with breast implant illness.
Sci Rep
January 2025
Section General Internal Medicine, Department of Internal Medicine, Amsterdam University Medical Centres, Amsterdam, The Netherlands.
Breast Implant Illness (BII) is characterized by a cluster of systemic and local symptoms affecting a subset of women with silicone breast implants. While symptom improvement is frequently observed following implant removal, the underlying mechanisms remain poorly understood, and the absence of reliable biomarkers complicates clinical decision-making. Here, we investigate inflammatory protein profiles in 43 women with BII, comparing pre- and post-explantation levels using the Olink Target 96 Inflammation panel and Meso Scale Discovery technology for absolute quantification.
View Article and Find Full Text PDFQuantitative Profiling pH Heterogeneity of Acidic Endolysosomal Compartments using Fluorescence Lifetime Imaging Microscopy.
Mol Biol Cell
January 2025
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147 USA.
The endo-lysosomal system plays a crucial role in maintaining cellular homeostasis and promoting organism fitness. The pH of its acidic compartments is a crucial parameter for proper function, and it is dynamically influenced by both intracellular and environmental factors. Here, we present a method based on fluorescence lifetime imaging microscopy (FLIM) for quantitatively analyzing the pH profiles of acidic endolysosomal compartments in diverse types of primary mammalian cells and in live organism .
View Article and Find Full Text PDFImmune responses in children with secondary infection of mycoplasma pneumoniae after COVID-19: focus on eosinophils and IgE.
BMC Infect Dis
January 2025
Luoyang Research Center for Inheritance and Innovation of Chinese Historical Civilization, Luoyang Institute of Science and Technology School of Marxism (LIT), No. 90 Wangcheng Avenue, Luolong District, Luoyang City, Henan Province, China.
Background: The COVID-19 (SARS-CoV-2) epidemic has posed a major challenge to global public health, especially in children. Some children may experience secondary infection with Mycoplasma pneumoniae after SARS-CoV-2 infection, which has attracted widespread attention. Studies have shown that eosinophils play an important role in respiratory tract infections and are involved in regulating immune responses and inflammatory processes.
View Article and Find Full Text PDFMultiplex Detection and Quantification of Virus Co-Infections Using Label-free Surface-Enhanced Raman Spectroscopy and Deep Learning Algorithms.
ACS Sens
January 2025
Department of Physics and Astronomy, Franklin College of Arts and Sciences, The University of Georgia, Athens, Georgia 30602, United States.
Multiple respiratory viruses can concurrently or sequentially infect the respiratory tract, making their identification crucial for diagnosis, treatment, and disease management. We present a label-free diagnostic platform integrating surface-enhanced Raman scattering (SERS) with deep learning for rapid, quantitative detection of respiratory virus coinfections. Using sensitive silica-coated silver nanorod array substrates, over 1.
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