MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of mRNAs. The swimming crab Portunus trituberculatus is one of the most important crustacean species for aquaculture in China. However, to date no miRNAs have been reported to for modulating growth in P. trituberculatus. To investigate miRNAs involved in the growth of this species, we constructed six small RNA libraries for big individuals (BIs) and small individuals (SIs) from a highly inbred family. Six mixed RNA pools of five tissues (eyestalk, gill, heart, hepatopancreas, and muscle) were obtained. By aligning sequencing data with those for known miRNAs, a total of 404 miRNAs, including 339 known and 65 novel miRNAs, were identified from the six libraries. MiR-100 and miR-276a-3p were among the most prominent miRNA species. We identified seven differentially expressed miRNAs between the BIs and SIs, which were validated using real-time PCR. Preliminary analyzes of their putative target genes and GO and KEGG pathway analyzes showed that these differentially expressed miRNAs could play important roles in global transcriptional depression and cell differentiation of P. trituberculatus. This study reveals the first miRNA profile related to the body growth of P. trituberculatus, which would be particularly useful for crab breeding programs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.margen.2016.03.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stress Response of the Juvenile Tri-Spine Horseshoe Crab Tachypleus tridentatus to Hypoxia: Insights From Phenotypic, Metabolomic, and Microbial Analyses.
Integr Zool
January 2025
Key Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai, China.
Over the past few decades, ocean hypoxia has been increasing due to human activities. Hypoxic stress, characterized by a reduced level of dissolved oxygen, is an escalating threat to marine ecosystems, with potentially devastating effects on the viability of endangered species such as the tri-spine horseshoe crab Tachypleus tridentatus. Even though this species is remarkably resilient to low oxygen levels, persistent hypoxia can negatively impact its population's survivability.
View Article and Find Full Text PDFNano-TiO impairs the health of crabs Charybdis japonica under warming conditions through waterborne and dietary exposures.
J Hazard Mater
January 2025
International Research Center for Marine Biosciences, Shanghai Ocean University, Ministry of Science and Technology, College of Fisheries and Life Science, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:
The widespread use of nano titanium dioxide (nano-TiO₂) poses ecological risks to marine ecosystems, especially when combined with ocean warming. However, most previous studies have only examined water-related exposures, leaving a gap in research on the impact of food transfer on organisms. In this work, the harmful impacts of nano-TiO on the Japanese swimming crab Charybdis japonica were studied through three scenarios: direct exposure (DE) of the crabs to warming and nano-TiO, indirect exposure (IE) via consumption of thick-shelled mussels Mytilus coruscus exposed to the same conditions, and combined exposure (CE), where crabs were directly subjected to warming and nano-TiO while feeding affected mussels.
View Article and Find Full Text PDFEdible portions of precooked blue swimming crab: Chemical composition and effect of chitooligosaccharide conjugate and high-pressure processing on microbial inactivation.
Food Chem X
January 2025
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Article Synopsis
- Analysis of blue swimming crab revealed that both meat and roe are high in protein, with roe containing more fat.
- The meat had higher levels of polyunsaturated fatty acids and distinct protein structures compared to the roe, which was primarily composed of leucine.
- The study demonstrated that techniques like chitooligosaccharide-catechin conjugate and high-pressure processing effectively reduced bacteria, improving food safety and quality for precooked crab products.
Tissue-specific vitellogenesis and 17β-estradiol facilitate ovarian maturation of the swimming crab Portunus trituberculatus.
Comp Biochem Physiol A Mol Integr Physiol
December 2024
Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Aquatic Animal Breeding Center of Shanghai University Knowledge Service Platform, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:
The present study investigated the changes of expression and localization of PtVg mRNA, tissue Vg/ Vn concentrations, the contents of progesterone and 17ß-estradiol during the ovarian development of P. trituberculatus. The results showed that: 1) The most abundant mRNA levels of PtVg were found in stage IV, and hepatopancreatic PtVg mRNA was markedly greater than that in ovaries from stage II to stage V.
View Article and Find Full Text PDFCharacterization of Myf6 and association with growth traits in swimming crab (Portunus trituberculatus).
BMC Genomics
December 2024
School of Life Sciences, Hebei University, Baoding, Hebei, 071000, China.
Background: Myogenic factor 6 (Myf6) plays an important role in muscle growth and differentiation. In aquatic animals and livestock, Myf6 contributes to improving meat quality and strengthening the accumulation of muscle flavor substances. However, studies on Myf6 gene polymorphisms in crustaceans have not been reported.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!