Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here, we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2, and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.
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http://dx.doi.org/10.3389/fpls.2016.00401 | DOI Listing |
Plant Dis
November 2023
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China.
Pear powdery mildew (PPM), caused by , is one of the most serious diseases affecting production in the Hebei pear-growing region of China. Iminoctadine trialbesilate and trifloxystrobin are known to have broad-spectrum activity against a wide range of plant pathogens, including . A total of 105 strains were isolated from 11 cities in Hebei Province from 2017 to 2019.
View Article and Find Full Text PDFMolecules
November 2022
Laboratory of Biochemistry, Biotechnologies and Health and Environment, Department of Biology, Faculty of Sciences, Ibn Tofail University, B.P. 133, Kenitra 14000, Morocco.
During the postharvest period, citrus fruits are exposed to , and . Pesticides such as imazalil (IMZ), thiabendazole (TBZ), orthophenylphenol (OPP), and guazatine (GUA) are commonly used as antifungals. Glyphosate (GP) is also used in citrus fields to eliminate weed growth.
View Article and Find Full Text PDFMicroorganisms
July 2022
Department of Biomedical Science, University of Wolverhampton, Wolverhampton WV1 1LY, UK.
In recent years, a rise in the number of contact lens users in the UK and worldwide coincided with an increased incidence of microbial keratitis. The aim of this study was to investigate the antimicrobial activities of polyhexamethylene guanidine (PHMG), polyaminopropyl biguanide (PAPB), and guazatine in comparison to the common contact lens disinfectant constituent, polyhexamethylene biguanide (PHMB). The study investigated these compounds against a broad range of organisms, including Acanthamoeba castellanii, Acanthamoeba polyphaga, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans.
View Article and Find Full Text PDFInt J Food Microbiol
May 2022
Programa Nacional de Investigación en Producción Citrícola, Instituto Nacional de Investigación Agropecuaria (INIA), Camino al Terrible s/n, Casilla postal: 68033, Salto, Uruguay. Electronic address:
Citrus sour rot caused by Geotrichum citri-aurantii is one of the most important postharvest diseases in citrus fruit, causing huge economic losses. Traditionally, it has been controlled by the postharvest application of guazatine and propiconazole fungicides, but restrictions in their use make it urgent to find an alternative for sour rot management. Natamycin, a common food preservative, and the organosulfuric compounds extracted from Allium species are safe food additives that control different foodborne pathogens.
View Article and Find Full Text PDFFood Microbiol
October 2021
ARC Training Centre for Food Safety in the Fresh Produce Industry, School of Life and Environmental Sciences, Sydney Institute of Agriculture, Faculty of Science, The University of Sydney, NSW, 2006, Australia. Electronic address:
Postharvest treatments with sanitizers and fungicides are applied to increase the quality, safety and shelf life of fresh produce including cantaloupes (also known as rockmelons). The primary role of sanitizers during cantaloupe washing is to prevent cross contamination of potentially pathogenic bacteria in washwater. Postharvest fungicide sprays or dips are employed to inhibit spoilage-causing fungi.
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