AI Article Synopsis

  • This study explored how low-frequency stimulation (LFS) affects the ability of CA1 neurons in guinea pig hippocampal slices to undergo long-term potentiation (LTP) after a high-frequency stimulation (HFS).
  • It was found that activating group I metabotropic glutamate receptors (mGluRs) prior to HFS suppresses LTP, as does the preconditioning with LFS.
  • The suppression of LTP can be reversed by blocking group I mGluRs or inositol 1,4,5-trisphosphate receptors (IP3Rs) right before HFS, indicating that mGluR activation following LFS interferes with LTP induction.

Article Abstract

We investigated the role of inositol 1,4,5-trisphosphate receptors (IP3Rs) activated by preconditioning low-frequency afferent stimulation (LFS) in the subsequent induction of long-term potentiation (LTP) in CA1 neurons in hippocampal slices from mature guinea pigs. Induction of LTP in the field excitatory postsynaptic potential or the population spike by the delivery of high-frequency stimulation (HFS, a tetanus of 100 pulses at 100 Hz) to the Schaffer collateral-commissural pathway to CA1 neuron synapses was suppressed when group I metabotropic glutamate receptors (mGluRs) were activated prior to the delivery of HFS. LTP induction was also suppressed when CA1 synapses were preconditioned 60 min before HFS by LFS of 1000 pulses at 1 Hz and this effect was inhibited when the test stimulation delivered at 0.05 Hz was either halted or applied in the presence of an antagonist ofN-methyl-d-aspartate receptors, group I mGluRs, or IP3Rs during a 20-min period from 20 to 40 min after the end of LFS. Furthermore, blockade of group I mGluRs or IP3Rs immediately before the delivery of HFS overcame the effects of the preconditioning LFS on LTP induction. These results suggest that, in CA1 neurons, after a preconditioning LFS, activation of group I mGluRs caused by the test stimulation results in IP3Rs activation that leads to a failure of LTP induction.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836634PMC
http://dx.doi.org/10.1101/lm.041053.115DOI Listing

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