The human genomic locus for the transcription factor TOX3 has been implicated in susceptibility to restless legs syndrome and breast cancer in genome-wide association studies, but the physiological role of TOX3 remains largely unknown. We found Tox3 to be predominantly expressed in the developing mouse brain with a peak at embryonic day E14 where it co-localizes with the neural stem and progenitor markers Nestin and Sox2 in radial glia of the ventricular zone and intermediate progenitors of the subventricular zone. Tox3 is also expressed in neural progenitor cells obtained from the ganglionic eminence of E15 mice that express Nestin, and it specifically binds the Nestin promoter in chromatin immunoprecipitation assays. In line with this, over-expression of Tox3 increased Nestin promoter activity, which was cooperatively enhanced by treatment with the stem cell self-renewal promoting Notch ligand Jagged and repressed by pharmacological inhibition of Notch signaling. Knockdown of Tox3 in the subventricular zone of E12.5 mouse embryos by in utero electroporation of Tox3 shRNA revealed a reduced Nestin expression and decreased proliferation at E14 and a reduced migration to the cortical plate in E16 embryos in electroporated cells. Together, these results argue for a role of Tox3 in the development of the nervous system.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbagrm.2016.04.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel Insights Into the Causal Effects and Shared Genetics Between Body Fat and Parkinson Disease.
CNS Neurosci Ther
November 2024
Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Aims: Existing observational studies examining the effect of body fat on the risk of Parkinson disease (PD) have yielded inconsistent results. We aimed to investigate this causal relationship at the genetic level.
Methods: We employed two-sample Mendelian randomization (TSMR) to investigate the causal effects of body fat on PD, with multiple sex-specific body fat measures being involved.
Goblet cell differentiation subgroups in colorectal cancer.
Proc Natl Acad Sci U S A
October 2024
Department of Oncology, University of Oxford, Oxford OX3 7DQ, United Kingdom.
The poor prognosis of relatively undifferentiated cancers has long been recognized, suggesting that selection against differentiation and in favor of uncontrolled growth is one of the most powerful drivers of cancer progression. Goblet cells provide the mucous surface of the gut, and when present in colorectal cancers (CRC), the cancers are called mucinous. We have used the presence of MUC2, the main mucous product of goblet cells, and an associated gene product, TFF3, to classify a large panel of nearly 80 CRC-derived cell lines into five categories based on their levels of MUC2 and TFF3 expression.
View Article and Find Full Text PDFUnderstanding genetic variations associated with familial breast cancer.
World J Surg Oncol
October 2024
Department of Surgical Oncology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Article Synopsis
- - Breast cancer is the most common cancer in women, with genetics accounting for 15-25% of cases, primarily involving mutations in genes like BRCA1 and BRCA2.
- - A comprehensive review of 768 articles identified 20 familial breast cancer-associated genes categorized by their penetrance levels and uncovered 10 genetic disorders related to the disease.
- - The National Comprehensive Cancer Network recommends genetic screening, focusing on high- and moderate-risk gene variations, and highlights the potential of next-generation sequencing (NGS) to uncover rare genetic links to breast cancer.
Exploring Genetic Interactions in Colombian Women with Polycystic Ovarian Syndrome: A Study on SNP-SNP Associations.
Int J Mol Sci
August 2024
Faculty of Sciences, Universidad Pedagógica y Tecnológica de Colombia, Tunja 150003, Colombia.
Article Synopsis
- Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women, identified as polygenic due to various single-nucleotide polymorphisms (SNPs).
- This study assessed the interactions between 27 identified SNPs to see how they contribute to the development of PCOS, using a sample of 49 women with PCOS and 49 controls.
- The findings suggested significant interactions among certain SNPs, hinting at the role of epistasis in PCOS development, but emphasized the need for larger studies to further investigate these interactions.
Tanshinone IIA from Salvia miltiorrhiza alleviates follicular maturation arrest symptoms in zebrafish via binding to the human androgen receptors and modulating Tox3 and Dennd1a.
Tissue Cell
June 2024
Toxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu 603203, India. Electronic address:
Follicular maturation arrest is a prevalent endocrine disorder characterized by hormonal imbalance, ovarian dysfunction, and metabolic disturbances leading to Polycystic ovarian syndrome (PCOS). Tanshinone IIA (TIIA), a bioactive compound derived from Salvia miltiorrhiza, has shown promising therapeutic potential in various diseases, including cardiovascular diseases and cancer. However, its effects on reproductive health and gynecological disorders, particularly PCOS, remain poorly understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!