AI Article Synopsis
- Laser capture microdissection (LCM) is a valuable technique in medical research, allowing the collection of specific cell populations for molecular analysis under a microscope.
- Adapting LCM procedures to fit different analysis platforms is crucial; this study focuses on refining LCM for preparing fresh frozen samples for NanoString analysis in a mouse mammary gland carcinoma model.
- The optimized LCM workflow yielded high-quality RNA samples suitable for NanoString, enabling precise detection of target genes and their related pathways, demonstrating the compatibility of NanoString with LCM.
Article Abstract
Laser capture microdissection (LCM) of tissue is an established tool in medical research for collection of distinguished cell populations under direct microscopic visualization for molecular analysis. LCM samples have been successfully analyzed in a number of genomic and proteomic downstream molecular applications. However, LCM sample collection and preparation procedure has to be adapted to each downstream analysis platform. In this present manuscript we describe in detail the adaptation of LCM methodology for the collection and preparation of fresh frozen samples for NanoString analysis based on a study of a model of mouse mammary gland carcinoma and its lung metastasis. Our adaptation of LCM sample preparation and workflow to the requirements of the NanoString platform allowed acquiring samples with high RNA quality. The NanoString analysis of such samples provided sensitive detection of genes of interest and their associated molecular pathways. NanoString is a reliable gene expression analysis platform that can be effectively coupled with LCM.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831786 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153270 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Investigating the hydrolysis of complex carbohydrates with salivary α-amylase.
Food Res Int
February 2025
Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark. Electronic address:
Currently, little is known about how complex carbohydrates (maltodextrins) with varying degrees of polymerisation (DP) and molecular branching interact with α-amylase in human saliva and the associated amounts and structures of generated reducing sugars. Therefore, this study aimed to investigate salivary α-amylase and the subsequent reducing sugars generated with complex carbohydrate stimuli. A secondary aim was to investigate reducing sugar generation and complex carbohydrate taste sensitivity.
View Article and Find Full Text PDFTackling antineoplastic drugs' contamination in healthcare settings: New insights on surface cleaning approaches.
J Occup Environ Hyg
January 2025
EPIUnit, Instituto de Saúde Pública, Universidade do Porto, Porto, Portugal.
Effective decontamination of hospital surfaces is crucial to protect workers from antineoplastic drugs (ADs) since dermal absorption is the main exposure route to these hazardous medicinal products. Sampling after daily cleaning in oncologic settings from a tertiary hospital was initially performed and exhibited low contamination levels; however, cyclophosphamide was still found (up to 957 pg/cm) above the guidance value (100 pg/cm) in four locations, evidencing the need to properly assess and update the cleaning protocols. Then, cleaning efficiencies of six solutions and different protocols were evaluated (including, for the first time, four commercial cleaning solutions/disinfectants not designed specifically for AD removal) after deliberate contamination of three model surfaces with 13 pharmaceuticals: bicalutamide, capecitabine, cyclophosphamide, cyproterone, doxorubicin, etoposide, flutamide, ifosfamide, imatinib, megestrol, mycophenolate mofetil, paclitaxel, and prednisone.
View Article and Find Full Text PDFAnti-inflammatory, immunostimulant and antimicrobial properties of tannic acid in the diet of Oreochromis niloticus infected with Aeromonas hydrophila.
Fish Shellfish Immunol
February 2025
Aquatic Organisms Health Laboratory (AQUOS), Aquaculture Department, UFSC, Rodovia Admar Gonzaga 1346, 88037-000, Florianópolis, SC, Brazil. Electronic address:
The study aimed to assess the impact of dietary supplementation with tannic acid on the growth, health, and survival of Oreochromis niloticus following exposure to Aeromonas hydrophila. A total of 320 fish were divided into 16 tanks and assigned to four treatment groups: feed with 0.2 % tannic acid (TA), 0.
View Article and Find Full Text PDFIn Situ Preparation of Silver Nanoparticles/Organophilic-Clay/Polyethylene Glycol Nanocomposites for the Reduction of Organic Pollutants.
Polymers (Basel)
December 2024
Catalysis Research Group (CRG), Department of Chemistry, College of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.
This work focuses on the preparation and application of silver nanoparticles/organophilic clay/polyethylene glycol for the catalytic reduction of the contaminants methylene blue (MB) and 4-nitrophenol (4-NP) in a simple and binary system. Algerian clay was subjected to a series of treatments including acid treatment, ion exchange with the surfactant hexadecyltrimethylammonium bromide (HTABr), immobilization of polyethylene glycol polymer, and finally dispersion of AgNPs. The molecular weight of polyethylene glycol was varied (100, 200, and 4000) to study its effect on the stabilization of silver nanoparticles (AgNPs) and the catalytic activity of the resulting samples.
View Article and Find Full Text PDFChronic exposure to liquid crystal monomer EBCN at environmentally relevant concentrations induces testicular dysfunction via the gut-testis axis.
J Hazard Mater
December 2024
Reproductive Medicine Center, Henan Provincial People's Hospital, Zhengzhou, China; Reproductive Medicine Center, Zhengzhou University People's Hospital, Zhengzhou, China; Henan Joint International Research Laboratory of Reproductive Bioengineering, Zhengzhou, China. Electronic address:
4-Cyano-4'-ethoxybiphenyl (EBCN) is a representative cyano liquid crystal monomer (LCM). While prior studies have documented the widespread occurrence of LCMs in diverse environmental and biological samples, research on their reproductive effects in vivo remains limited. This study employed 35-day and 70-day exposure models in mice to assess the short-term and long-term effects of environmentally relevant concentrations of EBCN on testicular health.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!