AI Article Synopsis
- * The study used gas chromatography with mass spectrometry (GC/MS-SIM) to accurately measure ergosterol and its esters in button mushrooms while implementing quality control to minimize errors.
- * Results showed that button mushrooms contain about 443 mg of ergosterol and 12 mg of esterified ergosterol per 100 g of dry matter, with ergosterol making up about 90% of the free sterol content and around 55% in the esterified fraction, which includes various other sterols.
Article Abstract
Ergosterol is the major sterol in button mushrooms (Agaricus bisporus) and can occur as free alcohol or esterified with fatty acids (ergosteryl esters). In this study, gas chromatography with mass spectrometry in the selected ion monitoring mode (GC/MS-SIM) was used to determine ergosterol and ergosteryl esters as well as other sterols and steryl esters in button mushrooms. Different quality control measures were established and sample preparation procedures were compared to prevent the formation of artifacts and the degradation of ergosteryl esters. The final method was then used for the determination of ergosterol (443 ± 44 mg/100 g dry matter (d.m.)) and esterified ergosterol (12 ± 6 mg/100 g d.m.) in button mushroom samples (n = 4). While the free sterol fraction was vastly dominated by ergosterol (∼90% of five sterols in total), the steryl ester fraction was more diversified (nine sterols in total, ergosterol ∼55%) and consisted primarily of linoleic acid esters.
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| http://dx.doi.org/10.1021/acs.jafc.6b00383 | DOI Listing |
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