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Modulating Enzyme's Conformational Space: Impact of Substrate Binding, Mode Alteration, and Active Site Mutation in DapC, an Aminotransferase Enzyme of Lysine Biosynthetic Pathway.
J Phys Chem B
January 2025
Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
The microbial aminotransferase enzyme DapC is vital for lysine biosynthesis in various Gram-positive bacteria, including . Characterization of the enzyme's conformational dynamics and identifying the key residues for ligand binding are crucial for the development of effective antimicrobials. This study employs atomistic simulations to explore and categorize the dynamics of DapC in comparison to other classes of aminotransferase.
View Article and Find Full Text PDFLow Pt Loading on Wolframite-Type NiWO to Excel the Electrocatalytic Water Splitting and Ammonia Oxidation Reaction.
ACS Appl Mater Interfaces
January 2025
Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Hydrogen production via water-splitting or ammonia electrolysis using transition metal-based electrodes is one of the most cost-effective approaches. Herein, ca. 1-4% of Pt atoms are stuffed into a wolframite-type NiWO lattice to improve the electrocatalytic efficiency.
View Article and Find Full Text PDFPersonalized Sleep Hygiene and Structured Rest Interventions Improve Sleep Quality and Energy in a Rotating Shift Worker: A Case Report.
Cureus
December 2024
Faculty of Medical Sciences, The University of the West Indies, St. Augustine, TTO.
A 38-year-old paint technician who worked rotating shifts reported mild sleep disruptions and increased fatigue. The company's medical staff reviewed his work patterns, rest habits, and home environment. They introduced a personalized sleep hygiene program and adjusted his break schedule, allowing short, structured rest periods.
View Article and Find Full Text PDFDeltopectoral approach without subscapularis detachment for reverse shoulder arthroplasty. Technique and results of a safe and reproducible subscapularis-sparing approach.
JSES Rev Rep Tech
February 2025
Clinique Claude Bernard, Unité de Chirurgie Orthopédique, Metz, France.
Background: The importance of the subscapularis for reverse total shoulder arthroplasty has been demonstrated, especially for internal rotation and stability. In a deltopectoral approach, a detachment of the subscapularis is performed (tenotomy, tuberosity peeling, or osteotomy), but the tendon is not always repairable at the end. When it is repaired, healing is obtained in only 40%-76% of the cases, with potential consequences for the outcomes.
View Article and Find Full Text PDFMedialization of shaft of humerus to provide medial buttress and prevent varus collapse in 3- and 4-part proximal humerus fractures: review of the surgical technique.
JSES Rev Rep Tech
February 2025
Department of Orthopaedics, Krishna Institute of Medical Sciences, Secunderabad, Telanagana, India.
Hypothesis: Proximal humerus fractures present a treatment challenge due to varied fracture configurations and a lack of consensus on optimal management. Locking plate designs offer promising solutions, yet technical guidelines for successful outcomes remain elusive. Complications are common, with fixation-related failures often attributed to varus collapse.
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