AI Article Synopsis
- Penicillium nordicum is a key fungus that produces ochratoxin A (OTA), a toxin that contaminates protein-rich foods, especially dry-cured meats.
- A study focused on the gene expression of a specific enzyme (otapksPN) involved in OTA production during a 30-day seasoning process of pork sausages.
- Results showed that both the expression of the otapksPN gene and OTA levels peaked around 10 days into the seasoning, with initial detection as early as 4 days but declining thereafter.
Article Abstract
Penicillium nordicum, an important and consistent producer of ochratoxin A (OTA), is a widely distributed contaminant of protein rich food with elevated NaCl. It is usually found on dry-cured meat products and is considered the main species responsible for their contamination by OTA. The aim of this work was to study the gene expression of a polyketide synthase (otapksPN) involved in P. nordicum OTA biosynthesis, and OTA production during a small-scale seasoning process. Fresh pork sausages were surface inoculated with P. nordicum and seasoned for 30days. Gene expression and OTA production were monitored throughout the seasoning process after 4, 5, 6, 7, 10, 14, and 30days. The expression of otapksPN gene was already detected after 4days and increased significantly after 7days of seasoning, reaching the maximum expression level after 10days (1.69×10(4)copies/100mg). Consistently with gene expression monitoring, OTA was detected from the 4th day and its content increased significantly from the 7th day, reaching the maximum level after 10days. In the late stages of the seasoning process, OTA did not increase further and the number of gene copies was progressively reduced after 14 and 30days.
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| http://dx.doi.org/10.1016/j.ijfoodmicro.2016.03.028 | DOI Listing |
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