Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.

J Microbiol Methods

Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address:

Published: June 2016

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.

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http://dx.doi.org/10.1016/j.mimet.2016.03.018DOI Listing

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