AI Article Synopsis
- - A carbapenem-resistant strain of *Pseudomonas aeruginosa*, known as NCGM1984, was identified in 2012 from a Japanese patient and showed the presence of IMP-type metallo-β-lactamase through immunochromatographic assays.
- - Genome sequencing revealed that NCGM1984 contained two copies of the *blaIMP-34* gene located on different sites of its chromosome, both part of a complex genetic structure with multiple associated genes.
- - The strain was classified as multilocus sequence typing ST235, noted as a high-risk clone, and the IMP-34 variant exhibited catalytic properties similar to IMP-1, hydrolyzing almost all tested β-lactam antibiotics
Article Abstract
A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all β-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824433 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0149385 | PLOS |
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A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.
PLoS One
August 2016
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, Japan.
Article Synopsis
- - A carbapenem-resistant strain of *Pseudomonas aeruginosa*, known as NCGM1984, was identified in 2012 from a Japanese patient and showed the presence of IMP-type metallo-β-lactamase through immunochromatographic assays.
- - Genome sequencing revealed that NCGM1984 contained two copies of the *blaIMP-34* gene located on different sites of its chromosome, both part of a complex genetic structure with multiple associated genes.
- - The strain was classified as multilocus sequence typing ST235, noted as a high-risk clone, and the IMP-34 variant exhibited catalytic properties similar to IMP-1, hydrolyzing almost all tested β-lactam antibiotics
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