AI Article Synopsis
- A miniaturized centrifugal platform is introduced for separating and analyzing biological components from small volume samples (~5 μL) using density centrifugation.
- The system effectively enriches leukocytes for microscopy visualization and recovers viable cells from gradient partitions, achieving high leukocyte extraction while removing 99.8% of red blood cells.
- The process for staining is simplified, requiring less time and expertise, making this platform a promising tool for medical diagnostics and research with its automated and cost-effective approach.
Article Abstract
We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824354 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153137 | PLOS |
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