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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein. | LitMetric

Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein.

J Vet Sci

Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.; Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.

Published: March 2016

AI Article Synopsis

Article Abstract

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808637PMC
http://dx.doi.org/10.4142/jvs.2016.17.1.119DOI Listing

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