AI Article Synopsis
- Researchers studied strains of Corynebacterium from eye patients in Japan, Belgium, and Switzerland, using various techniques like gene sequencing and mass spectrometry.
- They identified two new species, Corynebacterium lowii and Corynebacterium oculi, and updated the description of an existing species, Corynebacterium mastitidis.
- Type strains were designated for the new species, with specific DNA G+C content percentages measured through different methods for comparison.
Article Abstract
Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA-DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis. Type strains for these species are: C. lowii R-50085T (=LMG 28276T =CCUG 65815T) and C. oculi R-50187T (=LMG 28277T =CCUG 65816T). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085T, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187T and 67.8 % (HPLC) for C. mastitidis LMG 19040T [=S-8T =CCUG 38654T =CECT 4843T =CIP 105509T =DSM 44356T =IFO (NBRC)16160T =JCM 12269T].
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| http://dx.doi.org/10.1099/ijsem.0.001059 | DOI Listing |
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