AI Article Synopsis
- The study focused on creating a genetic linkage map for Porphyra yezoensis using intraspecific cross-experiments and CAPS analysis.
- Controlled and random crosses between male wildtype and female green mutant led to the production of conchocelis colonies, with specific DNA patterns indicating biparental inheritance.
- This research marks the first molecular confirmation of cross-fertilization in P. yezoensis and suggests that the methods used could benefit genetic studies in other similar macroalgae.
Article Abstract
As a part of the construction of a Porphyra yezoensis Ueda genetic linkage map, we conducted intraspecific cross-experiments and subsequent screening of cross-fertilized conchocelis by cleaved amplified polymorphic sequence (CAPS) analysis. The cross-experiments were carried out between males of the wildtype (KGJ) and females of the recessive green mutant (TU-2) using two methods, controlled and random crosses. A total of 42 and 186 wildtype-colored conchocelis colonies were obtained from the former and latter experiments, respectively. Among those, 49 DNA samples (14% and 23% obtained from the former and latter crosses, respectively) showed biparental CAPS patterns in the two gene regions (EF-1α open reading frame [ORF] region and V-ATPase). This study represents the first report in which the cross-fertilized conchocelis of P. yezoensis has been directly confirmed by molecular marker. The combination of the simple DNA extraction and CAPS analysis may be applicable in genetic studies of other macroalgae that are monoecious and/or grow slowly in laboratory culture.
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| http://dx.doi.org/10.1111/j.1529-8817.2008.00465.x | DOI Listing |
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