AI Article Synopsis
- There are 11 lytic transglycosylases in Pseudomonas aeruginosa that are involved in breaking down the bacterial cell wall for recycling and repair, but their specific functions are not fully known.
- Research reveals that SltB3 is an exolytic lytic transglycosylase that can recognize at least four sugars and can process a larger substrate made of 20 sugars, ultimately producing a specific compound.
- The study presents the first X-ray structure of SltB3, showing it has four domains arranged in a circular shape, allowing the peptidoglycan substrate to pass through while it is being processed.
Article Abstract
A family of 11 lytic transglycosylases in Pseudomonas aeruginosa, an opportunistic human pathogen, turn over the polymeric bacterial cell wall in the course of its recycling, repair, and maturation. The functions of these enzymes are not fully understood. We disclose herein that SltB3 of P. aeruginosa is an exolytic lytic transglycosylase. We characterize its reaction and its products by the use of peptidoglycan-based molecules. The enzyme recognizes a minimum of four sugars in its substrate but can process a substrate comprised of a peptidoglycan of 20 sugars. The ultimate product of the reaction is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid. The X-ray structure of this enzyme is reported for the first time. The enzyme is comprised of four domains, arranged within an annular conformation. The polymeric linear peptidoglycan substrate threads through the opening of the annulus, as it experiences turnover.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436585 | PMC |
| http://dx.doi.org/10.1021/acschembio.6b00194 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Examining the Biophysical Properties of the Inner Membrane of Gram-Negative ESKAPE Pathogens.
J Chem Inf Model
January 2025
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1C 5S7, Canada.
The World Health Organization has identified multidrug-resistant bacteria as a serious global health threat. Gram-negative bacteria are particularly prone to antibiotic resistance, and their high rate of antibiotic resistance has been suggested to be related to the complex structure of their cell membrane. The outer membrane of Gram-negative bacteria contains lipopolysaccharides that protect the bacteria against threats such as antibiotics, while the inner membrane houses 20-30% of the bacterial cellular proteins.
View Article and Find Full Text PDFAntibacterial properties of elastomers modified with chitosan.
Acta Bioeng Biomech
June 2024
2AGH University of Krakow, Faculty of Materials Science and Ceramics, Kraków, Poland.
Bacterial infections pose a serious threat to human health. For many years, there has been a search for materials that would inhibit their development. It was decided to take a closer look at various elastomeric materials with the addition of chitosan.
View Article and Find Full Text PDFChangeover method for biosafety cabinets using ozone gas.
PLoS One
January 2025
Center for Stem Cell and Regenerative Medicine, Institute of Science Tokyo, Bunkyo-ku, Tokyo, Japan.
This study evaluated the effectiveness of a biosafety cabinet equipped with an ozone generator, particularly during the transition periods between the production of cell products. As living cell products cannot undergo sterilization, maintaining an aseptic manufacturing environment is paramount. Raw materials, often derived from human tissues, are frequently contaminated with various resident bacteria, necessitating environmental resets after each process.
View Article and Find Full Text PDFObjective: Aim: To improve the results of treatment of patients with chronic wounds of the lower extremities by using complex treatment, including surgical interventions, VAC- therapy, as well as studying the effect of negative pressure on bacterial films of wounds, based on microbiological examination and immune-histochemical data.
Patients And Methods: Materials and Methods: During the period from 2019 to 2023 at the department's clinic, 68 patients with chronic wounds of the lower extremities were examined and treated. These are mainly women (n=63) aged from 35 to 80 years.
Bacterial vaginosis is associated with transcriptomic changes but not higher concentrations of cervical leukocytes in a study of women at high risk for HIV acquisition.
J Infect Dis
January 2025
Department of Medicine, University of Washington, Seattle, WA, USA.
Background: The association between bacterial vaginosis (BV) and increased HIV acquisition risk may be related to concentrations of HIV-susceptible immune cells in the cervix.
Methods: Participants (31 with BV and 30 with normal microbiota) underwent cervical biopsy at a single visit. Immune cells were quantified and sorted using flow cytometry (N=55), localization assessed by immunofluorescence (N=16), and function determined by bulk RNA sequencing (RNA-seq) of live CD45+ cells (N=21).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!