The definition of the number and nature of the signal transduction pathways involved in the pathogenesis and the identification of the molecules promoting metastasis spread might improve the knowledge of the natural history of osteosarcoma, also allowing refine the prognosis and opening the way to novel therapeutic strategies. Phosphatydil inositol (4,5) bisphosphate (PIP2), belonging to the Phosphoinositide (PI) signal transduction pathway, was related to the regulation of ezrin, an ezrin-radixin-moesin protein involved in metastatic osteosarcoma spread. The levels of PIP2 are regulated by means of the PI-specific Phospholipase C (PLC) enzymes. Recent literature data suggested that in osteosarcoma the panel of expression of PLC isoforms varies in a complex and unclear manner and is related to ezrin, probably networking with Ras GTPases, such as RhoA and Rac1. We analyzed the expression and the subcellular localization of PLC enzymes in cultured human osteosarcoma MG-63 cells, commonly used as an experimental model for human osteoblasts, using U-73122 PLC inhibitor, U-73343 inactive analogue, and by silencing ezrin. The treatment with U-73122 significantly reduces the number of MG-63 viable cells and contemporarily modifies the expression and the subcellular localization of selected PLC isoforms. U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving PI-specific Phospholipases C.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766154 | PMC |
| http://dx.doi.org/10.1186/s40064-016-1768-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Zuojin Pill enhances gastrointestinal motility by modulating the pacemaker potentials in interstitial cells of Cajal through multiple signaling pathways.
Int J Med Sci
December 2024
Department of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
Article Synopsis
- Zuojin Pill (ZJP) is a traditional herbal remedy aimed at treating gastrointestinal disorders, and the study explores how it affects interstitial cells of Cajal (ICCs) and gut movement in mice.
- The research found that ZJP depolarizes ICCs, reducing their pacemaker activity and affecting calcium signaling, which is crucial for normal GI motility; this process is influenced by specific receptor interactions and various signaling pathways.
- Additionally, ZJP improved intestinal transit in mouse models of GI motility disorders and increased levels of key substances that promote gut movement, suggesting its potential as a therapeutic agent in digestive health.
Induction of Ca signaling and cytotoxic responses of human lung fibroblasts upon an antihistamine drug oxatomide treatment and evaluating the protective effects of Ca chelating.
Fundam Clin Pharmacol
February 2025
Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Background: Oxatomide, an antihistamine drug of the diphenylmethylpiperazine family, has anti-inflammatory effects in airway disease. Because oxatomide was shown to cause diverse physiological responses in several cell models, the impact of oxatomide on Ca signaling and its related physiological effects has not been explored in IMR-90 human fetal lung fibroblasts.
Objectives: This study assessed the effect of oxatomide on cell viability and intracellular free Ca concentrations ([Ca]) and examined whether oxatomide-induced cytotoxicity through Ca signaling in IMR-90 cells.
Role of PLC/IP /IP R axis in excess molybdenum exposure induced apoptosis in duck renal tubular epithelial cells.
Environ Toxicol
January 2024
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, People's Republic of China.
Excess molybdenum (Mo) is harmful to animals, but its nephrotoxicity has not been comprehensively explained. To appraise the influences of excess Mo on Ca homeostasis and apoptosis via PLC/IP /IP R axis, primary duck renal tubular epithelial cells were exposed to 480 μM and 960 μM Mo, and joint of 960 μM Mo and 10 μM 2-APB or 0.125 μM U-73122 for 12 h (U-73122 pretreated for 1 h), respectively.
View Article and Find Full Text PDFMolybdenum and cadmium co-exposure induces CaMKKβ/AMPK/mTOR pathway mediated-autophagy by subcellular calcium redistribution in duck renal tubular epithelial cells.
J Inorg Biochem
November 2022
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.. Electronic address:
Excessive molybdenum (Mo) and cadmium (Cd) are toxic environmental pollutants. Our previous research confirmed excessive Mo and Cd co-induced calcium homeostasis disorder and autophagy in duck kidneys, but how calcium ion (Ca) regulates autophagy is unclear. The results revealed that the Mo- and/or Cd-induced cytosolic Ca concentration ([Ca]) increase mainly came from intracellular calcium stores.
View Article and Find Full Text PDFCadmium exposure induces autophagy via PLC-IP -IP R signaling pathway in duck renal tubular epithelial cells.
Environ Toxicol
November 2022
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Cadmium (Cd) is detrimental to animals, but nephrotoxic effects of Cd on duck have not been fully elucidated. To evaluate the impacts of Cd on Ca homeostasis and autophagy via PLC-IP -IP R pathway, primary duck renal tubular epithelial cells were exposed to 2.5 μM and 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!