Fasciolopsis buski is a food-borne zoonotic parasite which is transmitted by aquatic plants, with pigs and humans as the definitive hosts. The objective of the present study was to characterize the microRNA (miRNA) profiles of this parasite by Solexa deep sequencing and bioinformatic analysis. Approximately 12 million high-quality reads were obtained from adult F. buski. A total of 286 miRNA candidates were found and 24 miRNA candidates were conserved miRNAs in the miRBase database. Three novel miRNAs were identified and confirmed by stem-loop reverse transcriptase polymerase chain reaction (RT-PCR). The miRNAs found in the present study belong to 13 families whose members showed high bias. The guanine (G) was the dominant nucleotide at the beginning and middle of the conserved miRNAs, particularly at the positions of 2nd, 6th, and 13th. To our knowledge, this is the first report of the miRNA profiles of F. buski, which would lay a foundation for further functional studies of miRNAs of F. buski.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00436-016-4995-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fasciolopsis buski infection of the biliary tract: a case report.
Diagn Pathol
January 2025
Department of Pathology, Affiliated Hospital of Zunyi Medical University, Zunyi City, Guizhou Province, P.R. China.
Background: Fasciolopsis buski is a large fluke that parasitises the human small intestine, with its infection in the biliary tract being even rarer. Given its relatively rare occurrence in recent years, the clinical diagnosis of F. buski infections can pose certain challenges.
View Article and Find Full Text PDFRecognition of parasitic helminth eggs via a deep learning-based platform.
Front Microbiol
November 2024
Department of Human Parasitology, School of Basic Medicine Science, Hubei University of Medicine, Shiyan, China.
Introduction: Accurate and rapid diagnosis is crucial for the effective treatment of parasitosis. Traditional etiological methods, especially microscopic examination, are time-consuming, labor-intensive, and prone to false or missed detections. In response to these challenges, this study explores the use of artificial intelligence (AI) for the detection and classification of human parasite eggs through the YOLOv4 deep learning object detection algorithm.
View Article and Find Full Text PDFFasciolopsis buski isolated from human host, India has distinct clade based on nuclear ribosomal DNA sequences.
Indian J Med Microbiol
December 2024
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Electronic address:
Fasciolopsiasis caused by Fasciolopsis buski, is a significant cause of morbidity. In the present study F. buski detected during endoscopy of a 50-year-old male patient form Uttar Pradesh, India was used for phylogenetic analysis based on 28S rDNA and ITS2 regions.
View Article and Find Full Text PDFWestern Anthelmintics in Early Twentieth-Century China Colonial Practices and Knowledge on "Tropical Diseases" of the In/between.
Ber Wiss
December 2024
Chair of the History of Knowledge of Modern Societies, Helmut Schmidt University, Hamburg.
Protestant (medical) missionaries were the main proponents of Western medicine in China after the Opium Wars. Several studies have highlighted how they used spectacular surgery as a means of gaining public trust. As well as surgery, they also administered anthelmintic drugs such as santonin as a tool of persuasion and conversion.
View Article and Find Full Text PDFEpidemiology and Geographical Distribution of Human Trematode Infections.
Adv Exp Med Biol
July 2024
MediCheck Research Institute, Korea Association of Health Promotion, Seoul, Republic of Korea.
Article Synopsis
- - Digenetic trematodes are a large group of parasitic flatworms affecting humans, with over 109 species across 49 genera globally.
- - They are categorized based on their location in definitive hosts into six groups: blood flukes, liver flukes, lung flukes, heterophyid intestinal flukes, echinostome intestinal flukes, and miscellaneous intestinal flukes.
- - Notable species include the blood flukes Schistosoma, liver flukes like Fasciola and Clonorchis, and various types of intestinal flukes such as Metagonimus and Echinostoma.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!