Intermittent fasting decreases glucose and insulin levels and increases insulin sensitivity and lifespan. Decreased food intake influences the liver. Previous studies have shown gender differences in response to various types of caloric restriction, including every-other-day (EOD) feeding, in humans and rodents. Our goal was to show the influence of prolonged EOD feeding on the morphology, proliferation and apoptosis of livers from male and female mice. After nine months of an EOD diet, the livers from male and female mice were collected. We examined their morphology on histological slides using the Hematoxilin and Eosine (H_E) method and Hoechst staining of cell nuclei to evaluate the nuclear area of hepatocytes. We also evaluated the expression of mRNA for proto-oncogens, pro-survival proteins and apoptotic markers using Real Time Polimerase Chain Reaction (PCR). We noted increased lipid content in the livers of EOD fed female mice. EOD feeding lead to a decrease of proliferation and apoptosis in the livers of female and male mice, which suggest that tissue maintenance occurred during EOD feeding. Our experiment revealed sex-specific expression of mRNA for proto-oncogenes and pro-survival and pro-apoptotic genes in mice as well as sex-specific responses to the EOD treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808902 | PMC |
| http://dx.doi.org/10.3390/nu8030176 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fasting recovers age-related hypertension in the rats: reset of renal renin-angiotensin system components and klotho.
BMC Nephrol
December 2024
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Article Synopsis
- The study examined how intermittent fasting impacts age-related high blood pressure and the renal renin-angiotensin system in rats.
- Older rats showed higher blood pressure and hormone levels associated with hypertension compared to younger rats, as well as lower levels of α-klotho and kidney receptor proteins.
- Intermittent fasting, especially every other day fasting, effectively lowered blood pressure and improved certain RAS components in older rats, indicating its potential benefits for managing age-related hypertension.
How to Overcome a Snail? Identification of Putative Neurotoxins of Snail-Feeding Firefly Larvae (Coleoptera: Lampyridae, ).
Toxins (Basel)
June 2024
Institute of Zoology, University of Cologne, Zuelpicher Strasse 47b, 50674 Cologne, Germany.
The larvae of some lampyrid beetles are highly specialized predators of snails. They have been observed to climb on the shells of their prey and use this exposed position to bite and inject secretions potentially originating from the midgut. Besides serving the purpose of extra-oral digestion (EOD), injected compounds also seem to have a paralyzing effect.
View Article and Find Full Text PDFShort-term starvation at different feeding regimes on appetite responses, feeding utilization and physiological indices, of red hybrid tilapia () fingerlings reared in brackish water.
Heliyon
February 2024
Department of Aquaculture and Biotechnology, Faculty of Aquaculture and Marine Fisheries, Arish University, Arish, Egypt.
A 42 day factorial trial (3x2) was designed to evaluate the effect of short-term starvation with different feeding frequencies on performance, feed utilization, physiological status and appetite responses of red hybrid tilapia fingerlings. Eighteen plastic tanks with a capacity of (55 L) were used to accomplish this work. Fingerlings with an average initial weight of 23 g ± 0.
View Article and Find Full Text PDFDiet in phenotypically divergent sympatric species of African weakly electric fish (genus: Campylomormyrus)-A hybrid capture/HTS metabarcoding approach.
Mol Ecol
February 2024
Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany.
Article Synopsis
- - The study explores how
- Campylomormyrus
- , a mormyrid genus, evolved to have different species with variations in feeding structures and electric organ discharges (EOD), suggesting these changes are due to adapting to different food sources.
- - Researchers assessed the diets of five Campylomormyrus species and their closely related species Gnathonemus petersii, using advanced DNA techniques to analyze stomach contents, which included various invertebrates and vegetation.
- - Findings show that these species can access diverse food sources in their environments, primarily aquatic insect larvae, indicating that their physical differences in snout shape and EOD may help them exploit distinct diets, highlighting an evolutionary adaptation.
Every-other day (EOD) feeding regime decreases oxidative stress and inflammatory cascade in mouse liver: The immunohistochemical study.
Tissue Cell
December 2023
Department of Physiology Pomeranian Medical University in Szczecin, al. Powstancow Wlkp.72, 70-111 Szczecin, Poland.
Introduction: Positive effects of calorie restrictions (CR) and EOD include decreased body weight, prolonged life span, but also changes in metabolism of the liver. In present paper our aim was to examine antioxidative enzymes: Catalase (CAT) and Manganese superoxidative dismutase (MnSOD, SOD2) and Glutathione peroxidase 4 (Gpx-4) in connection to caspase-3 and inflammatory mediators (IL-1β and TNF- α) in EOD liver tissue in comparison to control mice.
Methods: After 9 months of EOD treatment male mouse liver tissue was harvested and prepared for analysis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!