AI Article Synopsis
- The study focused on using animal fleshing (ANFL) as a substrate to produce an extracellular protease enzyme from Clostridium limosum through advanced experimental design techniques.
- The optimal protease yield achieved was 433 U/ml, identifying the enzyme as an acidic metalloprotease with a molecular weight of 71 kDa, which is activated by metals like Zn(2+) and Mg(2+).
- The research demonstrated that the protease effectively breaks down ANFL during anaerobic fermentation, offering a sustainable method for managing tannery waste while producing a valuable enzyme for various industrial uses.
Article Abstract
In this study, animal fleshing (ANFL) was utilized as a substrate for the production of extracellular protease by Clostridium limosum through central composite rotatable design (CCRD) and response surface methodology (RSM). Optimum protease production of 433U/ml was achieved and the purified enzyme was identified as acidic metalloprotease, a monomeric protein. The molecular weight of the enzyme was 71kDa, whose activity was enhanced by bivalent metals such as Zn(2+) and Mg(2+). Scanning electron microscopy (SEM) examination also revealed the hydrolysis/microbial degradation of ANFL through protease activity in the anaerobic fermentation process. Simultaneous hydrolysis of ANFL and production of an enzyme with the potential for different industrial applications provide an attractive methodology for the disposal of tannery solid waste.
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| http://dx.doi.org/10.1016/j.biortech.2016.03.033 | DOI Listing |
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