AI Article Synopsis

  • Somatic embryogenesis is a process where new embryos are created from somatic tissues, useful for large-scale cloning and genetic engineering, while also providing insights into molecular changes during embryogenesis.
  • The study identified 1052 unique proteins in two embryogenic cell suspensions, describing their functions and creating two protein networks related to stress response and embryogenic capacity using Cytoscape.
  • This research offers the first extensive proteome analysis of embryogenic cell suspensions in coffee, aiding the understanding of somatic embryogenesis and enhancing protein annotation in this non-model crop, with all data accessible via ProteomeXchange (PXD002963).

Article Abstract

Somatic embryogenesis, is a process by which new viable embryos are produced from somatic tissues. Somatic embryogenesis is not only a useful biotechnological tool for the massive clonal propagation and genetic engineering but it also allows to obtain fundamental knowledge about the molecular changes that take place during embryogenesis. We present the proteome profile of two embryogenic cell suspensions. We identified 1052 non-redundant proteins. We present their known GO annotations and show two protein networks sharing the GO annotations related to stress and embryogenic capacity via the free program Cytoscape. To our knowledge these results give the first high-throughput proteome description of embryogenic cell suspensions and provide new information about somatic embryos for the whole plant community. The published proteome is a first step toward understanding somatic embryogenesis in coffee and toward a better annotation of proteins in an important non-model crop. All data are available via ProteomeXchange with identifier PXD002963.

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Source
http://dx.doi.org/10.1002/pmic.201500399DOI Listing

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