The cryostability of Saccharomyces cerevisiae cells decreased when they were cultivated under anaerobic conditions in a liquid growth medium YEPD as compared to the culture grown under aerobic conditions. The effect of cultivation conditions on the different cryostability of S. cerevisiae cells is discussed. The initial state of their bioenergetics was shown to influence the cryostability of yeast cells.
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The cryostability of Saccharomyces cerevisiae cells decreased when they were cultivated under anaerobic conditions in a liquid growth medium YEPD as compared to the culture grown under aerobic conditions. The effect of cultivation conditions on the different cryostability of S. cerevisiae cells is discussed.
View Article and Find Full Text PDF[Cryostability of yeast cells and their sensitivity to basic factors of freezing].
Mikrobiologiia
November 1989
The cryostability of Saccharomyces cerevisiae cells was found to correlate with their sensitivity to some physico-chemical factors of freezing. The threshold of their resistance to the main factors of cryoconservation (a low-temperature shock, the presence of a salt, a change in the pH of the medium) was determined at a temperature value physiological for this microorganism. This work was done in order to substantiate and predict optimal freezing regimes while making microbial cells pass into the state of deep cryptobiosis.
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