Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3',5,5'-tetramethylbenzidine-H2O2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40-6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 μM and 1.8 U/L, respectively. The half-maximal inhibition value IC50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2016.02.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A potential anti-Helicobacter pylori strategy: Exploring the antibacterial mechanism of organic acids in sea buckthorn (Hippophae rhamnoides L.).
Microbiol Res
March 2025
State Key Laboratory of Plateau Ecology and Agriculture, College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China. Electronic address:
Helicobacter pylori (H. pylori) infection is a highly prevalent causative agent of various gastric diseases. The search for natural alternatives to antibiotics that can effectively inhibit H.
View Article and Find Full Text PDFUrease-coupled systems and materials: design strategies, scope and applications.
J Mater Chem B
March 2025
Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Bangalore-562112, Karnataka, India.
Synthetic systems have co-opted urease, a crucial enzyme serving many biological functions, to recapitulate complex biological features. Therefore, the urease-urea feedback reaction network (FCRN) is reciprocated with soft materials to induce various animate-like features, including self-regulation, error correction, and decision-making capabilities, that are processed through a variety of non-linear functions. Although free-urease-based homogeneous systems are capable of adhering to many non-linear characteristics, they lack the ability to showcase the diffusion-controlled spatiotemporal phenomena.
View Article and Find Full Text PDFStructure difference of Jack bean urease and Helicobacter pylori urease on binding interactions with quercetin.
Int J Biol Macromol
March 2025
College of Food Science and Engineering, Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, Shandong Agricultural University, Taian, China. Electronic address:
Urease catalyzes the hydrolysis of urea to carbamate and ammonia, leading to nitrogen loss, environmental pollution, and health issues, so numerous compounds have been screened for urease inhibition using Jack bean urease (JBU) and H. pylori urease (HPU) without consideration their structure difference. Previous studies have shown that the same inhibitor can exhibit distinct inhibitory effects on JBU and HPU, but limited papers focus on the effects mechanism.
View Article and Find Full Text PDFFpnA, the Aspergillus fumigatus homolog of human ferroportin, mediates resistance to nickel, cobalt and gallium but does not function in iron homeostasis.
Commun Biol
March 2025
Institute of Molecular Biology, Biocenter, Medical University Innsbruck, Innsbruck, Austria.
Iron homeostasis is key to both the survival of virtually all organisms and the virulence of fungi including Aspergillus fumigatus, a human fungal pathogen causing life-threatening invasive infections. Unlike the extensively studied fungal species Saccharomyces cerevisiae and Schizosaccharomyces pombe, A. fumigatus encodes an uncharacterized homolog of vertebrate ferroportin (Fpn1), termed FpnA.
View Article and Find Full Text PDFUreases in nature: Multifaceted roles and implications for plant and human health - A review.
Int J Biol Macromol
March 2025
College of Chemistry, Fuzhou University, 350116 Fuzhou, China; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, 132101 Jilin, China. Electronic address:
Urease, a multifunctional enzyme that catalyzes the hydrolysis of urea into ammonia, plays a pivotal role in nitrogen metabolism across diverse organisms. While essential for survival, its unregulated activity is implicated in numerous pathologies, including peptic ulcers, nephropathy, and gastric cancer, as well as agricultural challenges such as soil ammonium depletion and reduced nitrogen-use efficiency. Beyond its canonical enzymatic function, urease engages in protein-protein interactions with bioactive counterparts like jaburetox, canatoxin, and soyuretox plant-derived proteins with insecticidal, antifungal, and membranolytic properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!