A new fluorescent probe 1 featuring one 1,8-naphthalimide and two [12]aneN3 units was synthesized. In the presence of Cu(2+) ions, the fluorescence emission of 1 was quenched by a factor of 127-fold and no interference by other metal ions was observed under physiological conditions. By means of titration and a Job's plot it was established that 1 forms a complex with Cu(2+) ions in a 1:2 ratio. The fluorescence of the 1-Cu(2+) complex was recovered by the addition of Adenosine-5'-triphosphate (ATP) in aqueous solution. Due to its low cytotoxicity, good water solubility, and high sensitivity, probe 1 was successfully applied in the sequential recognition of Cu(2+) and ATP in aqueous solution and HeLa cells. The highly selective and sensitive ability of 1-Cu(2+) complex to detect ATP even enables its bio-analytical applications in real-time imaging in living cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.talanta.2016.02.040 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Green synthesis of water-compatible layer-by-layer assembled sandwich bread-like molecularly imprinted polymers for selective recognition of baicalin from Scutellariae Radix.
J Chromatogr A
January 2025
Key Laboratory of Protection, Development and Utilization of Medicinal Resources in Liupanshan Area, Ministry of Education, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, China; Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China. Electronic address:
Molecularly imprinted polymers (MIPs) are typically synthesized in organic solvents, leading to poor compatibility with water, weak affinity and selectivity for target molecules in aqueous media. To address these challenges, a green and sustainable synthesis of sandwich bread-like ATP@MIP was conducted using polyethylenimide (PEI) and deep eutectic solvent (DES) as hydrophilic bi-functional monomers via layer-by-layer self-assembly on the attapulgite (ATP) carrier. The new ATP@MIP can provide a higher density of imprinting sites with more orderly and uniform distribution due to inhibiting the competitive polymerization between PEI and DES, thereby significantly enhancing recognition ability.
View Article and Find Full Text PDFAn expanded substrate scope for cross-chiral ligation enables efficient synthesis of long l-RNAs.
RSC Chem Biol
December 2024
Department of Chemistry, Texas A&M University College Station Texas 77843 USA
Despite the growing interest in mirror-image l-oligonucleotides, both as a robust nucleic acid analogue and as an artificial genetic polymer, their broader adoption in biochemical research and medicine remains hindered by challenges associated with the synthesis of long sequences, especially for l-RNA. Herein, we present a novel strategy for assembling long l-RNAs the joining of two or more shorter fragments using cross-chiral ligase ribozymes together with new substrate activation chemistry. We show that 5'-monophosphorylated l-RNA, which is readily prepared by solid-phase synthesis, can be activated by chemical attachment of a 5'-adenosine monophosphate (AMP) or diphosphate (ADP), yielding 5'-adenosyl(di- or tri-)phosphate l-RNA.
View Article and Find Full Text PDFAdvances of Stimuli-Responsive Amphiphilic Copolymer Micelles in Tumor Therapy.
Int J Nanomedicine
January 2025
Department of pharmacy, west china hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
Amphiphilic copolymers are composed of both hydrophilic and hydrophobic chains, which can self-assemble into polymeric micelles in aqueous solution via the hydrophilic/hydrophobic interactions. Due to their unique properties, polymeric micelles have been widely used as drug carriers. Poorly soluble drugs can be covalently attached to polymer chains or non-covalently incorporated in the micelles, with improved pharmacokinetic profiles and enhanced efficacy.
View Article and Find Full Text PDFAccurate DNA Sequence Prediction for Sorting Target-Chirality Carbon Nanotubes and Manipulating Their Functionalities.
ACS Nano
January 2025
South China Advanced Institute for Soft Matter Science and Technology, School of Emergent Soft Matter, South China University of Technology, Guangzhou 510640, China.
Synthetic single-wall carbon nanotubes (SWCNTs) contain various chiralities, which can be sorted by DNA. However, finding DNA sequences for this purpose mainly relies on trial-and-error methods. Predicting the right DNA sequences to sort SWCNTs remains a substantial challenge.
View Article and Find Full Text PDFCadmium adsorption onto aluminum hydroxide-modified attapulgite prepared with basic aluminum acetate.
Sci Rep
December 2024
College of Resources and Environment, Gansu Agricultural University, Lanzhou, 730070, China.
Cadmium pollution in water is becoming increasingly serious. Thus, the effective removal of Cd(II) from water has garnered attention. Aluminum hydroxide-modified attapulgite (ATP-AC) was prepared from basic aluminum acetate through a coprecipitation method that could efficiently adsorb Cd(II) in aqueous solution.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!