A procedure for quantitative estimation of lactoferrin is developed using the "sandwich" type of immunoenzymatic assay. Preparation of lactoferrin was purified from women colostrum by means of ion exchange chromatography; the sorbent (a ligand-containing complex of lactoferrin) was synthesized; the antibody conjugate against lactoferrin and peroxidase from horseradish was prepared; optimal conditions of the immunoenzymatic assay were selected. Concentration of lactoferrin in donor blood serum or plasma constituted 0.5-3.3 mg/ml; distinct alterations of the lactoferrin content were not found in blood of patients maintained on artificial circulation or in the blood used for transfusion.
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