AI Article Synopsis
- This study aimed to explore how static magnetic fields (SMFs) affect bone healing around titanium implants in rabbits using various analysis methods.
- The research involved 27 male New Zealand white rabbits, dividing them into six groups based on the presence of magnets and healing periods of 1, 4, and 8 weeks, leading to detailed observations of the effects on bone structure and gene expression.
- Results showed that SMFs significantly enhanced bone density and quality, promoted new bone growth, and activated several key genes and signaling pathways related to bone healing around the implants.
Article Abstract
Objectives: The aim of this study was to investigate the effects of static magnetic fields (SMFs) on bone regeneration around titanium implants by μCT, histologic analysis, microarrays, and quantitative real-time PCR (qRT-PCR).
Materials And Methods: Neodymium magnets provided the source of SMFs, the specimens were grade 5 titanium implants, and the animals were twenty-seven adult male New Zealand white rabbits. These implants were divided into six groups according to the presence of a magnet and predetermined healing period (1, 4, and 8 weeks). Each group comprised six specimens for μCT (n = 6) and histologic examination, and three specimens (n = 3) for microarrays and qRT-PCR, yielding a total of 54 specimens.
Results: The μCT data showed that SMFs increased bone volume fraction (bone volume/total volume, BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th). Histologic observation indicated that SMFs promoted new bone formation and direct bony contact with implants. Microarray analysis identified 293 genes upregulated (>twofold) in response to SMFs. The upregulated genes included extracellular matrix (ECM)-related genes (COL10A1, COL9A1, and COL12A1) and growth factor (GF)-related genes (CTGF and PDGFD), and the upregulation was confirmed by qRT-PCR. Gene Ontology (GO) and pathway analysis revealed the involvement of the mitogen-activated protein kinase (MAPK), Wnt, and PPAR-gamma signaling pathways in implant healing.
Conclusions: μCT, histology, microarrays, and real-time PCR indicate that SMFs could be an effective approach to improving bone regeneration around dental implants.
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| http://dx.doi.org/10.1111/clr.12812 | DOI Listing |
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