At least 30 different human proteins can fold abnormally to form the amyloid deposits that are associated with a number of degenerative diseases. The research presented here aimed at understanding the inhibitory potency of a food additive, brilliant blue FCF (BBF), on the amyloid fibril formation of lysozyme. Our results demonstrated that BBF was able to suppress the formation of lysozyme fibrils in a dose-dependent fashion. In addition, the structural features and conformational changes in the lysozyme samples upon the addition of BBF were further characterized using circular dichroism spectroscopy, nile red fluorescence spectroscopy, turbidity assay, and sodium dodecyl sulfate electrophoresis. Through molecular docking and molecular dynamics simulations, BBF's mechanism of action in lysozyme fibrillogenesis inhibition was found to be initiated by binding with the aggregation-prone region of the lysozyme. We believe the results from this research may contribute to the development of effective therapeutics for amyloidoses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2016.02.064 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Additive effect of mixtures of multiple strains combining the effects of single strains on human gut microbiota .
Biosci Microbiota Food Health
August 2024
Central Research Institute, Itoen Ltd., 21 Mekami, Sagara-cho, Haibara-gun, Shizuoka, Japan.
Probiotics exert their beneficial effects by improving the intestinal environment. Heat-inactivated probiotics may show similar effects. However, whether multi-strain mixtures (MSM) are better than single strains, irrespective of whether the bacteria are alive or dead, is unknown.
View Article and Find Full Text PDFDivergence of gut bacteria through the selection of genomic variants implicated in the metabolism of sugars, amino acids, and purines by small extracellular vesicles in milk.
Gut Microbes
December 2025
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Here, we report that small extracellular vesicles (sEVs) in milk mediate the communication between bacteria and animal kingdoms, increase the divergence of bacteria in the intestine, and alter metabolite production by bacteria. We show that bovine milk sEVs select approximately 55,000 genomic variants in 19 species of bacteria from the murine cecum . The genomic variants are transcribed into mRNA.
View Article and Find Full Text PDFDecoding consumers' interpretations of 'additive-free' and 'tobacco & water' cigarette advertising claims.
Tob Control
January 2025
Rutgers Institute for Nicotine & Tobacco Studies, New Brunswick, New Jersey, USA.
Objectives: In the USA, some tobacco companies replaced the marketing phrase '100% natural additive-free tobacco' with 'tobacco ingredients: tobacco & water' (T&W) after receiving warnings from the US Food and Drug Administration. This study assesses how people interpret the now-restricted additive-free claims and newer T&W claims on Natural American Spirit (NAS) and L&M cigarette packs.
Methods: An online between-subjects experiment randomised 2526 US adults to view one of three packs: an NAS additive-free pack, an NAS T&W pack or an L&M T&W pack.
Impact of CaNa2EDTA fortification on growth, antioxidant activity and stress-related gene expression in tilapia (Oreochromis niloticus) at different stocking densities paradigms.
PLoS One
January 2025
Department of Forestry and Natural Resources, Purdue University, West Lafayette, Indiana, United States of America.
Increasing aquaculture production requires high-density farming, which induces stress, necessitating supplements to mitigate its effects and ensure fish health. The aim of this study was to examine how CaNa2EDTA (EDTA) affects the growth, immune response and antioxidant activity in Nile tilapia (Oreochromis niloticus). The fish were raised at three different stocking densities: low (LD = 2.
View Article and Find Full Text PDFHigh glucose couples DJ-1 with PTEN to activate PDGFRβ for renal proximal tubular cell injury.
PLoS One
January 2025
VA Research, Education and Clinical Center, South Texas Veterans Health Care System, San Antonio, Texas, United States of America.
Article Synopsis
- The study investigates how high glucose levels in diabetes lead to kidney cell damage through the activation of a signaling pathway involving DJ-1 and PTEN.
- DJ-1 is found to be upregulated in kidney cells under high glucose conditions, which triggers the Akt/mTORC1 signaling pathway, resulting in cell growth and fibrosis.
- The research indicates that inhibiting DJ-1 can prevent glucose-induced cell growth and damage, while overexpressing DJ-1 replicates the harmful effects, highlighting its role in renal injury related to diabetes.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!