Objectives: The aim of this multicenter EUVAS study was to evaluate the diagnostic performance of multi-parametric indirect immunofluorescence (IIF) assays to detect anti-neutrophil cytoplasmic antibodies (ANCA) in granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA).
Patients And Methods: The study included 912 samples from diseased controls and 249 diagnostic samples from GPA (n=183) and MPA (n=66) patients. The performance of two automated multi-parametric assays [Aklides (Medipan/Generic Assays) and EuroPattern (Euroimmun)] combining IIF on cellular and purified antigen substrates was compared with two manual IIF analyses and with commercially available ELISAs for MPO- and PR3-ANCA (Euroimmun).
Results: The area under the curve (AUC) of the receiver operating characteristics (ROC) curve to discriminate AAV from controls was 0.925, 0.848, 0.855 and 0.904 for, respectively, the two manual analyses, Aklides and EuroPattern, and 0.959, 0.921 and 0.886 for, respectively, antigen-specific ELISA, antigen-coated beads, and microdot, respectively. Variation in pattern assignment between IIF methods was observed.
Conclusion: The performance of IIF depends on the substrate used and the definition of IIF patterns. The performance of automated IIF is improved by multi-parameter testing (combined IIF and antigen-specific testing). Given the variability between IIF methods, the diagnostic importance of this technique is questioned.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.autrev.2016.03.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Harmonization of anti-nuclear antibody testing (ANA) by indirect immunofluorescence assay: Results from ten years of UK NEQAS external quality assessment.
Clin Chim Acta
December 2024
Laboratory of Clinical Pathology, Azienda Sanitaria Universitaria Integrata, Udine, Italy.
External quality assurance (EQA) programs play a pivotal role in monitoring laboratory practices, allowing each laboratory to evaluate the consistency of results across different methods as well the ability of individual laboratories to compare and improve over time their own performance. The objective of our study was to analyze the UK NEQAS EQA reports for the "Antibodies to Nuclear and Related Antigens" program from 2013 to 2023, to assess the overall level of harmonization of the responses for anti-nuclear antibody (ANA) testing by indirect immunofluorescence (IIF), in terms of both pattern and titer consensus. As a second aim, we analyzed the impact of the introduction in UK NEQAS EQA reports of the International Consensus on ANA Patterns (ICAP) nomenclature and of digital image recognition on the harmonization of the ANA HEp-2 IIF test.
View Article and Find Full Text PDFRadiomics for differential diagnosis of Bosniak II-IV renal masses via CT imaging.
BMC Cancer
December 2024
Department of Urology, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, 100191, P.R. China.
Rationale And Objectives: The management of complex renal cysts is guided by the Bosniak classification system, which may be inadequate for risk stratification of patients to determine the appropriate intervention. Radiomics models based on CT imaging may provide additional useful information.
Materials And Methods: A total of 322 patients with Bosniak II-IV cysts were included in the study from January 2010 to December 2019.
Comparison of BIOCHIP Mosaic Based Indirect Immunofluorescence and Oral Direct Immunofluorescence in Patients with Oral Mucosal Pemphigus.
Indian Dermatol Online J
October 2024
Department of Pathology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
Background: Oral mucosal lesions in pemphigus vulgaris may precede cutaneous lesions and can cause diagnostic confusion. Diagnosis can be made by histopathology, direct immunofluorescence (DIF), and indirect immunofluorescence (IIF). DIF of the oral mucosa is an invasive procedure and difficult to perform in patients with severe mucosal ulcer, and studies have shown that BIOCHIP-IIF can be used to detect desmoglein 1 and 3 in the serum of patients with pemphigus.
View Article and Find Full Text PDFAutomatic classification of HEp-2 specimens by explainable deep learning and Jensen-Shannon reliability index.
Artif Intell Med
November 2024
Department of Electronic Engineering, University of Rome Tor Vergata, via del Politecnico 1, 00133 Rome, Italy; Interdisciplinary Center for Advanced Studies on Lab-on-Chip and Organ-on-Chip Applications (ICLOC), University of Rome Tor Vergata, 00133 Rome, Italy.
The Anti-Nuclear Antibodies (ANA) test using Human Epithelial type 2 (HEp-2) cells in the Indirect Immuno-Fluorescence (IIF) assay protocol is considered the gold standard for detecting Connective Tissue Diseases. Computer-assisted systems for HEp-2 image analysis represent a growing field that harnesses the potential offered by novel machine learning techniques to address the classification of HEp-2 images and ANA patterns. In this study, we introduce an innovative platform based on transfer learning with pre-trained deep learning models.
View Article and Find Full Text PDFClinicopathological characteristics of patients with low titer anti-phospholipase A2 receptor antibodies verified by indirect immunofluorescence assay.
Clin Chim Acta
December 2024
Nephrology Division, Peking Union Medical College Hospital, No 1, Shuaifuyuan, Wangfujing St, Beijing 100730, PR China.
Article Synopsis
- This study investigates the diagnostic accuracy of indirect immunofluorescence (IIF) for assessing anti-phospholipase A2 receptor antibodies (PLA2R-abs) in patients with inconclusive grey-zone results from enzyme-linked immunosorbent assay (ELISA) related to primary membranous nephropathy (pMN).
- Among 52 patients with grey-zone results, those diagnosed with pMN had significantly higher levels of PLA2R-abs and lower serum creatinine than those with non-pMN conditions.
- The PLA2R-IIF showed a sensitivity of 72% and specificity of 88%, indicating it could enhance diagnostic confidence in grey-zone patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!