AI Article Synopsis
- Identifying poor-quality tissue-engineered oral mucosa grafts is essential for successful clinical use in regenerative medicine.
- This study explored the impact of β-D-xylopyranoside-n-propane-2-one (XPP) on oral epithelial regeneration using a 3D oral mucosa model.
- XPP treatment improved epithelial thickness and consistency, boosted key marker expressions like decorin and syndecan-1, and activated Akt/mTOR signaling, suggesting it enhances the quality of the oral mucosa epithelium.
Article Abstract
Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting. This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration. Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling. Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness. It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates. These findings suggest that XPP supplementation contributes to consistent epithelial regeneration. Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
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| http://dx.doi.org/10.1080/09168451.2016.1153957 | DOI Listing |
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