AI Article Synopsis
- A novel approach was developed to create a quantum dot (QD) probe conjugated with RGD peptides, aimed at targeting pancreatic carcinoma cells for imaging.
- The QD-RGD probe effectively marked pancreatic cancer cells in vitro, showing promising results through fluorescence and confocal microscopy techniques.
- The probe exhibited low toxicity and strong optical properties, suggesting its potential for early detection of pancreatic cancer.
Article Abstract
Background/aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of pancreatic carcinoma cells.
Methods: Pancreatic carcinoma cells were routinely cultured and marked with QD-RGD probe. The QD-RGD probe on the fluorescence-labeled cancer cell was observed by fluorescence microscopy and laser confocal microscopy. Cancer cell viability was detected by MTT assay after culturing with QD-RGD probe.
Results: Fluorescence microscopy and laser confocal microscopy displayed that 10nmol/L QD-RGD probe was able to effectively mark pancreatic carcinoma cells. In comparison with organic dyes and fluorescent proteins, the quantum dot-RGD probe had unique optical and electronic properties.
Conclusion: QD-RGD probe has a low cytotoxicity with an excellent optical property and biocompatibility. These findings support further evaluation of QD-RGD probes for the early detection of pancreatic cancer.
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| http://dx.doi.org/10.1159/000443062 | DOI Listing |
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